Invitrogen

USP16 Polyclonal Antibody

USP16 Antibody in Immunocytochemistry (ICC/IF)

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USP16 Antibody (PA5-104195) in ICC/IF

Immunofluorescent analysis of USP16 in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with USP16 polyclonal antibody (Product # PA5-104195) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

USP16 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-104195

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human USP16(Accession Q9Y5T5), corresponding to amino acid residues K140-P190.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2853523

产品详细信息

Antibody detects endogenous levels of total USP16.

靶标信息

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Deubiquitinating enzyme 16; deubiquitinating enzyme 16 {ECO:0000255|HAMAP-Rule:MF_03062}; Ubiquitin carboxyl-terminal hydrolase 16; ubiquitin carboxyl-terminal hydrolase 16 {ECO:0000255|HAMAP-Rule:MF_03062}; ubiquitin specific protease 16; Ubiquitin thioesterase 16; ubiquitin thioesterase 16 {ECO:0000255|HAMAP-Rule:MF_03062}; ubiquitin thiolesterase 16; Ubiquitin-processing protease UBP-M; ubiquitin-specific processing protease 16; Ubiquitin-specific-processing protease 16; ubiquitin-specific-processing protease 16 {ECO:0000255|HAMAP-Rule:MF_03062}; USP16

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基因别名: 1200004E02Rik; 2810483I07Rik; 6330514E22Rik; MSTP039; UBP-M; UBPM; USP16

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UniProt ID: (Human) Q9Y5T5, (Rat) Q2KJ09, (Mouse) Q99LG0

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Entrez Gene ID: (Human) 10600, (Rat) 288306, (Mouse) 74112

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