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USP44 Antibody (PA5-143988) in ICC/IF
Immunocytochemistry analysis of USP44 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in USP44 Polyclonal antibody (Product # PA5-143988) using a dilution of 4 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
产品信息
PA5-143988
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human USP44 recombinant protein (Position: R211-S712).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3075202
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
靶标信息
Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP44 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes.[supplied by OMIM, Mar 2008].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Deubiquitinating enzyme 44; inactive deubiquitinating enzyme 44; inactive ubiquitin carboxyl-terminal hydrolase 44; inactive ubiquitin thioesterase 44; inactive ubiquitin-specific-processing protease 44; Ubiquitin carboxyl-terminal hydrolase 44; ubiquitin specific peptidase 49 pseudogene; ubiquitin specific protease 44; Ubiquitin thioesterase 44; ubiquitin thiolesterase 44; Ubiquitin-specific-processing protease 44
基因别名: E430004F17Rik; USP44
UniProt ID: (Human) Q9H0E7, (Mouse) Q8C2S0
Entrez Gene ID: (Human) 84101, (Rat) 314746, (Mouse) 327799
mitotic nuclear division
protein deubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
cell division
regulation of spindle checkpoint
regulation of mitotic cell cycle spindle assembly checkpoint
negative regulation of ubiquitin protein ligase activity
biological_process
regulation of proteasomal protein catabolic process
proteolysis
ubiquitin-dependent protein catabolic process
cell cycle
