Invitrogen

Ubiquitin B Polyclonal Antibody

1 Published Figure
1 Reference
View all (6) Ubiquitin B antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-95195

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (IHC)

-
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Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human Ubiquitin recombinant protein (Position: M77-G152).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807000

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Caco-2 whole cell, human K562 whole cell, human THP-1 whole cell, rat PC-12 whole cell, rat brain tissue, mouse brain tissue. IHC: rat intestine tissue, human mammary cancer tissue, mouse intestine tissue. ICC/IF: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: epididymis secretory protein Li 50; HUBCEP52; MGC127041; polyubiquitin B; Polyubiquitin-B; RPL40; ubiquitin C

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Gene Aliases: AL033289; HEL-S-50; Rps27a; Uba52; UBB; Ubb2; UBC

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UniProt ID: (Human) P0CG47, (Mouse) P0CG49, (Rat) P0CG51

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Entrez Gene ID: (Human) 7314, (Mouse) 22187, (Rat) 192255

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Function(s)
protein binding
Process(es)
male meiosis I female meiosis I male gonad development female gonad development ubiquitin homeostasis hypothalamus gonadotrophin-releasing hormone neuron development mitochondrion transport along microtubule neuron projection morphogenesis regulation of mitochondrial membrane potential adipose tissue development fat pad development regulation of proteasomal protein catabolic process seminiferous tubule development energy homeostasis regulation of neuron death positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity protein polyubiquitination nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding stimulatory C-type lectin receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway glycogen biosynthetic process transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest transforming growth factor beta receptor signaling pathway Notch signaling pathway I-kappaB kinase/NF-kappaB signaling JNK cascade fibroblast growth factor receptor signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death Wnt signaling pathway endosomal transport macroautophagy viral life cycle virion assembly translesion synthesis negative regulation of transforming growth factor beta receptor signaling pathway anaphase-promoting complex-dependent catabolic process positive regulation of protein ubiquitination regulation of type I interferon production negative regulation of type I interferon production tumor necrosis factor-mediated signaling pathway nucleotide-excision repair, DNA incision ion transmembrane transport TRIF-dependent toll-like receptor signaling pathway interstrand cross-link repair NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway error-prone translesion synthesis DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability cellular protein metabolic process innate immune response positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia nucleotide-binding oligomerization domain containing signaling pathway global genome nucleotide-excision repair error-free translesion synthesis intracellular transport of virus negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of signal transduction by p53 class mediator positive regulation of protein monoubiquitination
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