Invitrogen

Ubiquitin C Polyclonal Antibody

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2 篇参考文献

查看其他4个Ubiquitin C抗体

抗体检测数据 (3)
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Ubiquitin C Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ubiquitin C Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Ubiquitin C Antibody (PA5-76144) in IHC (P)

Immunohistochemistry analysis of Ubiquitin C in paraffin-embedded human liver. Samples were incubated with Ubiquitin C polyclonal antibody (Product # PA5-76144) at a dilution of 1:100. Results were magnified with a 40x lens.

Ubiquitin C Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ubiquitin C Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ubiquitin C Antibody in Western Blot (WB)

Ubiquitin C Antibody in Western Blot (WB)

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Ubiquitin C Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Ubiquitin C in paraffin-embedded human liver. Samples were incubated with Ubiquitin C polyclonal antibody (Product # PA5-76144) at a dilution of 1:100. Results were magnified with a 40x lens.

Ubiquitin C Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Ubiquitin C in paraffin-embedded human breast carcinoma. Samples were incubated with Ubiquitin C polyclonal antibody (Product # PA5-76144).

Ubiquitin C Antibody in Western Blot (WB) — Western blot analysis of Ubiquitin C. Samples were incubated with Ubiquitin C polyclonal antibody (Product # PA5-76144).

Ubiquitin C Antibody in Western Blot (WB) — Effect of silencing enolase 1 and ubiquitin C. CM = conditioned medium, CN = control (no CM treatment), beta-cat = beta-catenin plasmids, siEno1 = Enolase 1 siRNA, siUbc = ubiquitin C siRNA, EO = EO771 mammary tumor cells, TR = TRAMP prostate cancer cells, and PA = PANC-1 pancreas cancer cells. The double asterisk indicates p < 0.01. (A) siRNA-mediated knockdown of enolase 1, and ubiquitin C in EO771 breast cancer cells. (B&C) Promotion of MTT-based proliferation, and scratch-based migration of EO771 breast cancer cells by enolase 1 and ubiquitin C siRNA-treated CMs in 2 days. (D-I) Effects of enolase 1 and ubiquitin C siRNAs. Silencing these two proteins significantly prevented the reduction in EdU-based proliferation and Transwell invasion of EO771, TRAMP, and PANC-1 cells by their own beta-catenin-overexpressing iTS CMs in 2 days.

产品信息

PA5-76144

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

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免疫组化（石蜡） (IHC (P))

1:50-1:100

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide of human Poly-Ubiquitin.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2719871

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Osteosarcoma-enriched transcripts paradoxically generate osteosarcoma-suppressing extracellular proteins.

eLife

Li K,Huo Q,Dimmitt NH,Qu G,Bao J,Pandya PH,Saadatzadeh MR,Bijangi-Vishehsaraei K,Kacena MA,Pollok KE,Lin CC,Li BY,Yokota H

PA5-76144 was used in Western Blotting to support the possibility of employing a paradoxical strategy of utilizing Osteosarcoma transcriptomes for the treatment of Osteosarcoma.

Tue Mar 21 00:00:00 UTC 2023

Generation of the tumor-suppressive secretome from tumor cells.

Theranostics

Liu S,Sun X,Li K,Zha R,Feng Y,Sano T,Dong C,Liu Y,Aryal UK,Sudo A,Li BY,Yokota H

PA5-76144 was used in Western Blot to study whether the anti-tumor secretomes can be produced from tumor cells.

Fri Jan 28 00:00:00 UTC 2022

Generation of the tumor-suppressive secretome from tumor cells.

Theranostics

Liu S,Sun X,Li K,Zha R,Feng Y,Sano T,Dong C,Liu Y,Aryal UK,Sudo A,Li BY,Yokota H

PA5-76144 was used in Western Blot to study whether the anti-tumor secretomes can be produced from tumor cells.

Fri Jan 28 00:00:00 UTC 2022

生物信息学

蛋白别名: polyubiquitin C; Polyubiquitin-C

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基因别名: 2700054O04Rik; AI194771; HMG20; Rps27a; TI-225; Uba52; Ubb; UBC

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UniProt ID: (Human) P0CG48, (Mouse) Q29120, (Rat) Q63429

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Entrez Gene ID: (Human) 7316, (Mouse) 22190, (Rat) 50522

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protease binding protein binding poly(A) RNA binding

ubiquitin homeostasis ubiquitin-dependent protein catabolic process G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity protein polyubiquitination nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding stimulatory C-type lectin receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway glycogen biosynthetic process transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest transforming growth factor beta receptor signaling pathway Notch signaling pathway I-kappaB kinase/NF-kappaB signaling JNK cascade fibroblast growth factor receptor signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death Wnt signaling pathway endosomal transport macroautophagy viral life cycle virion assembly translesion synthesis negative regulation of transforming growth factor beta receptor signaling pathway anaphase-promoting complex-dependent catabolic process regulation of type I interferon production negative regulation of type I interferon production tumor necrosis factor-mediated signaling pathway nucleotide-excision repair, DNA incision ion transmembrane transport TRIF-dependent toll-like receptor signaling pathway interstrand cross-link repair NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway error-prone translesion synthesis DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability cellular protein metabolic process innate immune response positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia nucleotide-binding oligomerization domain containing signaling pathway global genome nucleotide-excision repair error-free translesion synthesis intracellular transport of virus negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of signal transduction by p53 class mediator