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V5 Tag Antibody (MA5-15253-D488) in ICC/IF
Immunofluorescent analysis of V5 Tag was performed using 70% confluent V5-H3-6XHis transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with V5 Tag Monoclonal Antibody (E10/V4RR), DyLight 488 (Product # MA5-15253-D488) at 5 µg/mL and Histone H3 Recombinant Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then label... View More
产品信息
MA5-15253-D488
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
E10/V4RR
抗原
GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
偶联物
激发/发射光谱
492/519 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with proprietary stabilizer
内含物
0.02% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2537641
产品详细信息
MA5-15253-D488 has been successfully used for immunofluorescence. DyLight 488 has an excitation/emission of 493/518 nm.
靶标信息
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag
