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VAChT Antibody (600-401-MK7) in ICC/IF
Immunofluorescence of Rabbit Anti-VAChT Antibody. Cell Line: Post-natal rat pup (PND1) heterogeneous brain cells. Primary Antibody: Rabbit Anti-VAChT Antibody at 15µg/mL overnight at 4°C. Counterstain Antibody: Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated (p/n 611-741-127) at 5µg/mL for 1hr at RT. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Nuclear Counter Stain: DAPI. Image: A) DAPI (blue). B) Anti-VAChT (green) and Secondary. C) Merge of A+B images. D) Secondary only. Localization: predicted membrane, Golgi apparatus... View More
产品信息
600-401-MK7
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-VAChT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of rat vesicular acetylcholine transporter conjugated to Keyhole Limpet Hemocyanin (KLH).
偶联物
形式
Liquid
浓度
0.9 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against rat VAChT. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and human.
靶标信息
This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MGC12716; solute carrier family 18 (vesicular acetylcholine transporter), member 3; solute carrier family 18 (vesicular acetylcholine), member 3; solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3; solute carrier family 18, member 3; VAChT; Vesicular acetylcholine transporter
基因别名: rVAT; SLC18A3; VACHT; Vat
UniProt ID: (Human) B2R7S1, (Rat) Q62666
Entrez Gene ID: (Human) 6572, (Rat) 60422
