Invitrogen

VAMP2 Recombinant Rabbit Monoclonal Antibody (JM11-00)

VAMP2 Antibody in Immunocytochemistry (ICC/IF)

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VAMP2 Antibody (MA5-32606) in ICC/IF

Immunofluorescence analysis of VAMP2 (Vesicle-associated membrane protein 2) was performed using 70% confluent log phase U-87 MG and Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with VAMP2 Recombinant Rabbit Monoclonal Antibody (JM11-00) (Product # MA5-32606) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) High... View More

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产品信息

MA5-32606

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:50-1:200

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JM11-00

抗原

Synthetic peptide within Human VAMP2 aa 9-42

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809883

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Synaptobrevin; synaptobrevin 2; Synaptobrevin 2 (vesicle-associated membrane protein VAMP-2); synaptobrevin II; Synaptobrevin-2; vamp; VAMP-2; vesicle-associated membrane 2; Vesicle-associated membrane protein (synaptobrevin 2); Vesicle-associated membrane protein 2; vesicle-associated membrane protein 2 (synaptobrevin 2)

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基因别名: RATVAMPB; RATVAMPIR; SYB; Syb-2; SYB2; sybII; VAMP-2; VAMP2

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UniProt ID: (Human) P63027, (Mouse) P63044, (Rat) P63045

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Entrez Gene ID: (Human) 6844, (Mouse) 22318, (Rat) 24803

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