Invitrogen

VAMP3 Polyclonal Antibody

VAMP3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

VAMP3 Antibody (PA1-767A) in ICC/IF

Immunofluorescent analysis of VAMP3 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a VAMP3 polyclonal antibody (Product # PA1-767A) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjug... View More

Product Image Gallery

VAMP3 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of VAMP3 was observed in Mouse Brain and Rat Brain in comparison to Mouse Liver and Rat Liver using VAMP3 Polyclonal Antibody (Product # PA1-767A) in western blot. {RE}

产品信息

PA1-767A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

1:10-1:100

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Sythetic peptide corresponding to residues M(1) S T G V P S G S S A A T G S N R R(18) C of mouse VAMP-3.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2288259

产品详细信息

PA1-767A detects vesicle-associated membrane protein 3 (VAMP-3)/cellubrevin in human, rat and mouse samples.

PA1-767A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~13 kDa protein representing VAMP-3 in HeLa cell lysates.

PA1-767A immunogen is a sythetic peptide corresponding to residues M(1) S T G V P S G S S A A T G S N R R(18) C of mouse VAMP-3. This immunizing peptide (Cat. # PEP-145) is available for use in neutralization and control experiments.

靶标信息

Synaptobrevins/VAMPs, syntaxins, and the 25-kDa synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Single bioengineered ncRNA molecule for dual-targeting toward the control of non-small cell lung cancer patient-derived xenograft tumor growth.

Biochemical pharmacology

Petrek H,Yan Ho P,Batra N,Tu MJ,Zhang Q,Qiu JX,Yu AM

PA1-767A was used in Western Blotting to simultaneously introduce two miRNAs into non-small cell lung cancer cells by using single recombinant "combinatorial BERA" (CO-BERA) molecule.

Thu Jul 01 00:00:00 UTC 2021

Recycling endosomes attach to the trans-side of Golgi stacks in Drosophila and mammalian cells.

Journal of cell science

Fujii S,Kurokawa K,Inaba R,Hiramatsu N,Tago T,Nakamura Y,Nakano A,Satoh T,Satoh AK

PA1-767A was used in Immunocytochemistry to indicate that fly and mammalian recycling endosomes are organelles that are equivalent to trans-Golgi networks in plants.

Wed Feb 26 00:00:00 UTC 2020

SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate trafficking of membrane type 1-matrix metalloproteinase (MT1-MMP) during invadopodium formation and tumor cell invasion.

Molecular biology of the cell

Williams KC,McNeilly RE,Coppolino MG

PA1-767A was used in western blot to study the trafficking of MT1-MMP to invadopodia during tumor invasion and the roles played by SNAP23, syntaxin-4 and VAMP7

Tue Jul 01 00:00:00 UTC 2014

Characterization of VAMP-2 in the lung: implication in lung surfactant secretion.

Cell biology international

Wang P,Howard MD,Zhang H,Chintagari NR,Bell A,Jin N,Mishra A,Liu L

PA1-767A was used in Western Blotting to characterise the distribution of vesicle-associated membrane proteins in rat lung and alveolar type II cells.