Invitrogen

VAV1 Monoclonal Antibody (2E11)

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VAV1 Antibody (MA5-17198) in WB

Western blot was performed using Anti-VAV1 Monoclonal Antibody (2E11) (Product # MA5-17198) and a 98kDa band corresponding to VAV1 was observed in Raji, MOLT-4, Jurkat, THP-1 but not in T-47D and MCF7 which are reported to be negative. Whole cell extracts (30ug) of Raji (Lane 1), MOLT-4 (Lane 2), Jurkat (Lane 3), THP-1 (Lane 4), T-47D (Lane 5) and MCF7 (Lane 6) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dr... View More

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VAV1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of SUFU was observed in Raji, MOLT-4, Jurkat, THP-1, and not in T-47D and MCF7 using VAV1 Monoclonal Antibody (2E11) (Product # MA5-17198) in western blot. {RE}

产品信息

MA5-17198

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:250

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2E11

抗原

Purified recombinant fragment of human VAV1 (amino acids: 121-324) expressed in E. Coli.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538669

产品详细信息

MA5-17198 targets VAV1 in FACS, indirect ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17198 immunogen is purified recombinant fragment of human VAV1 (amino acids: 121-324) expressed in E. Coli.

MA5-17198 detects VAV1 which has a predicted molecular weight of approximately 98.3kDa.

靶标信息

This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Filgotinib suppresses HIV-1-driven gene transcription by inhibiting HIV-1 splicing and T cell activation.

The Journal of clinical investigation

Yeh YJ,Jenike KM,Calvi RM,Chiarella J,Hoh R,Deeks SG,Ho YC

MA5-17198 was used in Western Blotting to identify HIV-1-suppressing agents that can inhibit HIV-1 reactivation and reduce HIV-1-induced immune activation.