Bethyl Laboratories

VAV3 Polyclonal Antibody

VAV3 Antibody in Immunoprecipitation (IP)

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VAV3 Antibody (A303-833A) in IP

Detection of human VAV3 by western blot of immunoprecipitation. Samples: Whole cell lysate (50 µg for input; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-VAV3 antibody (Product # A303-833A) used for WB at 1 µg/mL and used for IP at 6 µg/mg lysate. VAV3 was also immunoprecipitated by rabbit anti-VAV3 antibody BL13957, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 3 minutes.

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产品信息

A303-833A

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,500

免疫沉淀 (IP)

2-10 µg/mg lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 200 and 250 of Human vav 3 guanine nucleotide exchange factor.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

phosphate/tris citrate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

靶标信息

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。