Invitrogen

VCAM-1 (CD106) Polyclonal Antibody

VCAM-1 (CD106) Antibody in Immunocytochemistry (ICC/IF)

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VCAM-1 (CD106) Antibody (PA5-86042) in ICC/IF

Immunocytochemical analysis of VCAM-1 (CD106) in SHG-44 cells using a VCAM-1 (CD106) Polyclonal antibody (Product # PA5-86042) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

VCAM-1 (CD106) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VCAM-1 (CD106) Antibody in Western Blot (WB)

VCAM-1 (CD106) Antibody in Flow Cytometry (Flow)

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产品信息

PA5-86042

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:5,000

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免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

流式细胞分析 (Flow)

1:50-1:100

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Hamster, Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide within Human VCAM1aa 1-50

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% Glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2802843

靶标信息

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Nobiletin and xanthohumol counteract the TNFα-mediated activation of endothelial cells through the inhibition of the NF-κB signaling pathway.

Cell biology international

Corrado C,Barreca MM,Raimondo S,Diana P,Pepe G,Basilicata MG,Conigliaro A,Alessandro R

PA5-86042 was used in Western Blot to highlight that xanthohumol and nobiletin can counteract the effects of TNF-α on angiogenesis and invasiveness, mainly through Vascular Endothelial Growth Factor and NF-κB pathways.

Wed Mar 01 00:00:00 UTC 2023

Unveiling the CHO surfaceome: Identification of cell surface proteins reveals cell aggregation-relevant mechanisms.

Biotechnology and bioengineering

Klingler F,Mathias S,Schneider H,Buck T,Raab N,Zeh N,Shieh YW,Pfannstiel J,Otte K

PA5-86042 was used in Flow cytometry/Cell sorting to explore the CHO surfaceome by enriching exposed N-glycosylated membrane proteins before tandem mass spectrometry (MS/MS) analyses, identifying a total of 449 surface proteins, including 34 proteins specific for production cells.

Sun Aug 01 00:00:00 UTC 2021

生物信息学

蛋白别名: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1; VCAM-1

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基因别名: CD106; INCAM-100; Vcam-1; VCAM1; VCAM1B

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UniProt ID: (Human) P19320, (Rat) P29534

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Entrez Gene ID: (Human) 7412, (Rat) 25361, (Mouse) 22329

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功能

integrin binding primary amine oxidase activity cell adhesion molecule binding

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