Invitrogen

VE-cadherin Monoclonal Antibody (BV9)

VE-cadherin Antibody in Immunocytochemistry (ICC/IF)

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VE-cadherin Antibody (MA1-198) in ICC/IF

Immunofluorescent analysis of VE-Cadherin (green) in Huvec cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a VE-Cadherin mouse monoclonal antibody (Product # MA1-198) at a concentration of 3 µg/mL in blocking buffer for 1 hou... View More

VE-cadherin Antibody in Western Blot (WB)

VE-cadherin Antibody in Flow Cytometry (Flow)

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产品信息

MA1-198

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

1-5 µg/mL

流式细胞分析 (Flow)

0.5-2 µg/mL

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

BV9

抗原

Recombinant protein corresponding to the extracellular domain of human VE-Cadherin.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2633308

产品详细信息

MA1-198 VE-Cadherin antibody detects human VE-cadherin by western blot, immunofluorescence and flow cytometry. This monoclonal antibody recognizes the extracellular domain of the VE-cadherin protein which is highly expressed at intercellular junctions of vascular endothelial cells such as Huvec.

靶标信息

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

iTRAQ Proteomics Identified the Potential Biomarkers of Coronary Artery Lesion in Kawasaki Disease and In Vitro Studies Demonstrated That S100A4 Treatment Made HCAECs More Susceptible to Neutrophil Infiltration.

International journal of molecular sciences

Weng KP,Chien KJ,Huang SH,Huang LH,Lin PH,Lin Y,Chang WH,Chen CY,Li SC

MA1-198 was used in Western Blotting to conclude that S100A4 treatment resulted in an incompact endothelial layer and made HCAECs more susceptible to in vitro neutrophil infiltration.

Sun Oct 23 00:00:00 UTC 2022

Changes in Human Foetal Osteoblasts Exposed to the Random Positioning Machine and Bone Construct Tissue Engineering.

International journal of molecular sciences

Mann V,Grimm D,Corydon TJ,Krüger M,Wehland M,Riwaldt S,Sahana J,Kopp S,Bauer J,Reseland JE,Infanger M,Mari Lian A,Okoro E,Sundaresan A

MA1-198 was used in Western Blotting to investigate the effects of simulated microgravity on biological processes and to engineer 3D bone constructs.

Mon Mar 18 00:00:00 UTC 2019

Cholesterol crystals increase vascular permeability by inactivating SHP2 and disrupting adherens junctions.

Free radical biology & medicine

Mani AM,Chattopadhyay R,Singh NK,Rao GN

Published figure using VE-cadherin monoclonal antibody (Product # MA1-198) in Western Blot

Wed Aug 01 00:00:00 UTC 2018

Microgravity Affects Thyroid Cancer Cells during the TEXUS-53 Mission Stronger than Hypergravity.

International journal of molecular sciences

Kopp S,Krüger M,Bauer J,Wehland M,Corydon TJ,Sahana J,Nassef MZ,Melnik D,Bauer TJ,Schulz H,Schütte A,Schmitz B,Oltmann H,Feldmann S,Infanger M,Grimm D

MA1-198 was used in Western Blotting to examine the effects of short-term microgravity on poorly differentiated follicular thyroid cancer cells resulting from 6 min of exposure to microgravity on a sounding rocket flight.

Wed Dec 12 00:00:00 UTC 2018

生物信息学

蛋白别名: 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; Vascular endothelial cadherin; VE-cadherin

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基因别名: 7B4; CD144; CDH5

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UniProt ID: (Human) P33151

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Entrez Gene ID: (Human) 1003

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功能

receptor binding calcium ion binding protein binding beta-catenin binding protein phosphatase binding ion channel binding cadherin

参与通路

blood vessel maturation cell-cell junction assembly homophilic cell adhesion via plasma membrane adhesion molecules transforming growth factor beta receptor signaling pathway negative regulation of cell proliferation single organismal cell-cell adhesion adherens junction organization positive regulation of establishment of endothelial barrier regulation of establishment of cell polarity