Invitrogen

VEGF-165 Recombinant Rabbit Monoclonal Antibody (R010)

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VEGF-165 Antibody in Neutralization (Neu)

VEGF-165 Antibody in Neutralization (Neu)

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VEGF-165 Antibody (MA5-52280) in Neu

Neutralization (cell proliferation assay) of VEGF-165 in human umbilical vein endothelial cells (HUVEC). Samples were incubated with VEGF-165 monoclonal antibody (Product # MA5-52280). The ED50 for this effect is typically 10-47 ng/mL in the presence of 10 ng/mL human VEGF165.

VEGF-165 Antibody in Neutralization (Neu)

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VEGF-165 Antibody in Neutralization (Neu) — Neutralization (cell proliferation assay) of VEGF-165 in human umbilical vein endothelial cells (HUVEC). Samples were incubated with VEGF-165 monoclonal antibody (Product # MA5-52280). The ED50 for this effect is typically 10-47 ng/mL in the presence of 10 ng/mL human VEGF165.

产品信息

MA5-52280

应用

建议稀释比

已发表文章

酶联免疫吸附实验 (ELISA)

1:250-1:2,000

-

中和实验 (Neu)

Assay-dependent

-

产品规格

种属反应

Cynomolgus monkey, Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

R010

抗原

Recombinant Human/Cynomolgus VEGF165/VEGFA protein (Ala 27-Arg191)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

100 µg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Cross-reactivity in ELISA Mouse VEGF164 and Rat VEGF164.

靶标信息

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: H-VEGF-165; L-VEGF; M-VEGF-165; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular endothelial growth factor isoform 165/endothelial-specific mitogen; Vascular permeability factor; VEGF; VEGF165; VPF

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基因别名: MVCD1; simVEGF165; VEGF; VEGFA; VPF

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UniProt ID: (Human) H3BLW7

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Entrez Gene ID: (Human) 7422, (Cynomolgus monkey) 102140241

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fibronectin binding cytokine activity platelet-derived growth factor receptor binding vascular endothelial growth factor receptor binding protein binding growth factor activity heparin binding chemoattractant activity identical protein binding protein homodimerization activity vascular endothelial growth factor receptor 1 binding vascular endothelial growth factor receptor 2 binding protein heterodimerization activity receptor agonist activity extracellular matrix binding growth factor

negative regulation of transcription from RNA polymerase II promoter angiogenesis ovarian follicle development patterning of blood vessels vasculogenesis response to hypoxia in utero embryonic development kidney development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation positive regulation of receptor internalization platelet degranulation positive regulation of leukocyte migration heart morphogenesis outflow tract morphogenesis coronary vein morphogenesis nervous system development mesoderm development lactation positive regulation of cell proliferation regulation of cell shape positive regulation of endothelial cell migration positive regulation of gene expression monocyte differentiation macrophage differentiation lung development positive regulation of cell migration regulation of cGMP metabolic process epithelial cell differentiation positive regulation of vascular endothelial growth factor receptor signaling pathway post-embryonic camera-type eye development positive regulation of protein complex assembly positive regulation of protein autophosphorylation activation of protein kinase activity positive regulation of CREB transcription factor activity positive regulation of peptidyl-serine phosphorylation tube formation endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus lymph vessel morphogenesis positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway VEGF-activated neuropilin signaling pathway growth eye photoreceptor cell development positive regulation of tyrosine phosphorylation of Stat3 protein negative regulation of apoptotic process surfactant homeostasis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway cell maturation camera-type eye morphogenesis cardiac muscle fiber development branching morphogenesis of an epithelial tube positive regulation of axon extension involved in axon guidance artery morphogenesis positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of positive chemotaxis induction of positive chemotaxis positive regulation of cellular component movement positive regulation of cell division positive regulation of focal adhesion assembly primitive erythrocyte differentiation mammary gland alveolus development cardiac vascular smooth muscle cell development coronary artery morphogenesis regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of transcription from RNA polymerase II promoter in response to hypoxia cellular response to hypoxia dopaminergic neuron differentiation commissural neuron axon guidance positive regulation of protein kinase C signaling positive regulation of cell migration involved in sprouting angiogenesis positive regulation of branching involved in ureteric bud morphogenesis regulation of retinal ganglion cell axon guidance positive regulation of peptidyl-tyrosine autophosphorylation positive regulation of p38MAPK cascade positive regulation of histone deacetylase activity positive regulation of retinal ganglion cell axon guidance positive regulation of protein kinase D signaling