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VEGFB Antibody (500-P267-1MG) in WB
Western Blot: To detect Human VEGF-B by Western Blot analysis VEGFB Polyclonal Antibody (Product # 500-P267-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human VEGF-B is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P267-1MG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human VEGF-B
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930153
产品详细信息
AA Sequence of recombinant protein: PVSQPDAPGH QRKVVSWIDV YTRATCQPRE VVVPLTVELM GTVAKQLVPS CVTVQRCGGC CPDDGLECVP TGQHQVRMQI LMIRYPSSQL GEMSLEEHSQ CECRPKKKDS AVKPDSPRPL CPRCTQHHQR PDPRTCRCRC RRRSFLRCQG RGLELNPDTC RCRKLRR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF-B. Anti-Human VEGF-B-specific antibody was purified by affinity chromatography employing an immobilized Human VEGF-B matrix.
Sandwich ELISA: To detect Human VEGF-B by sandwich ELISA (using 100 µL/well) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human VEGF-B (500-P267BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human VEGF-B.
Western Blot: To detect Human VEGF-B by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human VEGF-B is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.
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生物信息学
蛋白别名: Vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VRF
基因别名: VEGFB; VEGFL; VRF
UniProt ID: (Human) P49765
Entrez Gene ID: (Human) 7423
