Invitrogen

VMP1 Polyclonal Antibody

VMP1 Antibody in Immunocytochemistry (ICC/IF)

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VMP1 Antibody (PA5-77784) in ICC/IF

Immunofluorescent analysis of VMP1 in human colon carcinoma cell line (RKO). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with VMP1 polyclonal antibody (Product # PA5-77784) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Rabbit 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: A) DAPI nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) VMP1 Antibody, D) Merged image. Magnification: 60x.

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产品信息

PA5-77784

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide from the C-terminal of Human VMP1 (aa 391-402)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2736786

产品详细信息

A 1:1,000 dilution of PA5-77784 was sufficient for detection of VMP1 in 15 µg of Human 293T Cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 46 kDa.

靶标信息

VMP1 is a stress-induced protein that, when overexpressed, promotes formation of intracellular vacuoles followed by cell death. May be involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis.VMP1 is a transmembrane protein that co-localizes with LC3, a marker of the autophagosomes. It interacts with beclin-1, a mammalian autophagy initiator, through the VMP1-Atg domain, which is essential for autophagosome formation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。