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VPS16 Antibody (PA5-100086) in ICC/IF
Immunofluorescent analysis of VPS16 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and VPS16 polyclonal antibody (Product # PA5-100086) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-100086
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human VPS16(Accession Q9H269), corresponding to amino acid residues V330-V380.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2815616
产品详细信息
Antibody detects endogenous levels of total VPS16.
靶标信息
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hVPS16; mVPS16; RP11-12M19.2; vacuolar protein sorting 16 homolog; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog; VPS16 CORVET/HOPS core subunit
基因别名: 1810074M16Rik; hVPS16; VPS16
UniProt ID: (Human) Q9H269
Entrez Gene ID: (Human) 64601, (Mouse) 80743, (Rat) 296159
