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VPS18 Antibody (MA5-37854) in WB
Western blot analysis of VPS18 in extracts of various cell lines. Samples were incubated with VPS18 Monoclonal antibody (Product # MA5-37854) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
产品信息
MA5-37854
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
ARC2156
抗原
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VPS18 (Q9P253)
偶联物
形式
Liquid
浓度
0.31 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2897774
产品详细信息
Positive Samples: HeLa, Mouse lung, Mouse brain, Rat brain
Immunogen sequence: MASILDEYEN SLSRSAVLQP GCPSVGIPHS GYVNAQLEKE VPIFTKQRID FTPSERITSL VVSSNQLCMS LGKDTLLRID LGKANEPNHV ELGRKDDAKV
靶标信息
VPS18 is a vesicle mediated protein that plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hVPS18; vacuolar protein sorting 18 homolog; vacuolar protein sorting protein 18; Vacuolar protein sorting-associated protein 18 homolog; VPS18 CORVET/HOPS core subunit
基因别名: 9930024E13Rik; KIAA1475; PEP3; VPS18
UniProt ID: (Human) Q9P253, (Mouse) Q8R307
Entrez Gene ID: (Human) 57617, (Mouse) 228545, (Rat) 296083
