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VPS34 Antibody (PA5-35215) in ICC/IF
Immunocytochemistry analysis of VPS34 in U251 cells. Samples were incubated with VPS34 polyclonal antibody (Product # PA5-35215) using a dilution of 1:200 for 2 hr at room temperature followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:1,000 for 1hr. Cells were treated with Chloroquine (50 μM, 16hr), then fixed with 4% PFA (20 min), and permeabilized with Triton X-100 (0.2%, 30 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min). PI3KC3 immunoreactivity is localized to aut... View More
产品信息
PA5-35215
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH conjugated synthetic peptide between 14-39 amino acids from human PI3KC3
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2552525
产品详细信息
This antibody is predicted to react with mouse, porcine, rat and Xenopus based on sequence homology.
靶标信息
PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: catalytic subunit type 3 Antibody; Class III PI 3-kinase; hVps34; hVps34 Antibody; OTTMUSP00000023341; OTTMUSP00000023350; phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; phosphatidylinositol 3-kinase, catalytic subunit type 3; Phosphoinositide-3-kinase class 3; phosphoinositide-3-kinase, class 3; PI3-kinase type 3; PI3K type 3; pi3kc3; PIK3C3 Antibody; PtdIns-3-kinase type 3
基因别名: hVps34; PIK3C3; VPS34
UniProt ID: (Human) Q8NEB9
Entrez Gene ID: (Human) 5289
autophagosome assembly
cytokinesis
protein phosphorylation
phosphatidylinositol biosynthetic process
endosome organization
macroautophagy
protein processing
regulation of cytokinesis
toll-like receptor 9 signaling pathway
phosphatidylinositol-3-phosphate biosynthetic process
cellular response to glucose starvation
response to leucine
early endosome to late endosome transport
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
regulation of protein secretion
