Invitrogen

VPS34 Polyclonal Antibody

VPS34 Antibody in Immunocytochemistry (ICC/IF)

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VPS34 Antibody (PA5-85581) in ICC/IF

Immunofluorescence analysis of Phosphatidylinositol 3-kinase catalytic subunit type 3 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with VPS34 Polyclonal Antibody (Product # PA5-85581) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody,... View More

VPS34 Antibody in Immunoprecipitation (IP)

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VPS34 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across Reh, A375, HT1080, U2OS, HT-29, PC3, Hep G2. owing to their inherent genetic constitution. Relative expression of Phosphatidylinositol 3-kinase catalytic subunit type 3 was observed in Reh, A375, HT1080, HT-29 and PC3 but not in U2OS and Hep G2 using Anti-VPS34 Polyclonal Antibody (Product # PA5-85581) in Western Blot. {RE}

产品信息

PA5-85581

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the N-terminus region of human VPS34.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2792721

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (86%), Xenopus laevis (92%), Dog (99%), Pig (98%), Bovine (99%).

Positive Control: 293T, A431, HeLa, HepG2, U87-MG, SK-N-SH, IMR32, SK-N-AS, unboiled hVps34-transfected 293T cells.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: catalytic subunit type 3 Antibody; Class III PI 3-kinase; hVps34; hVps34 Antibody; OTTMUSP00000023341; OTTMUSP00000023350; phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; phosphatidylinositol 3-kinase, catalytic subunit type 3; Phosphoinositide-3-kinase class 3; phosphoinositide-3-kinase, class 3; PI3-kinase type 3; PI3K type 3; pi3kc3; PIK3C3 Antibody; PtdIns-3-kinase type 3

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基因别名: hVps34; PIK3C3; VPS34

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UniProt ID: (Human) Q8NEB9

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Entrez Gene ID: (Human) 5289

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功能

protein kinase activity protein binding ATP binding 1-phosphatidylinositol-3-kinase activity kinase

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