Invitrogen

VPS53 Polyclonal Antibody

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VPS53 Antibody (PA5-118356) in WB

Western Blot using VPS53 Polyclonal Antibody (Product # PA5-118356) at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa. Observed band size: 95 kDa.

VPS53 Antibody in Immunoprecipitation (IP)

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产品信息

PA5-118356

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫沉淀 (IP)

0.5-2 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived Human VPS53 fragment

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7

内含物

0.03% ProClin 300

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2902950

靶标信息

The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.

仅用于科研。不用于诊断过程。未经明确授权不得转售。