Invitrogen

Villin Polyclonal Antibody

Villin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

Villin Antibody (PA5-22072) in ICC/IF

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Villin antibody (Product # PA5-22072) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

产品信息

PA5-22072

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 7 and 246 (P09327) of Villin

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.85 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11155190

产品详细信息

Recommended positive controls: HepG2, mouse kidney, rat kidney.

Predicted reactivity: Mouse (89%), Rat (89%), Bovine (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin-4 expression in the colonic epithelium.

FEBS open bio

Kawaguchi M,Kataoka H,Kiwaki T,Weiting L,Nagata S,Kitamura K,Fukushima T

PA5-22072 was used in Immunohistochemistry-immunofluorescence to investigated the effects of adrenomedullin (AM) on junctional molecules in primary-cultured murine intestinal epithelial cells and discovered that AM upregulates claudin-4 expression.

Sat Apr 01 00:00:00 UTC 2023

A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia.

iScience

Zheng B,Ko KP,Fang X,Wang X,Zhang J,Jun S,Kim BJ,Luo W,Kim MJ,Jung YS,Cervantes CL,Park JI

PA5-22072 was used in Immunohistochemistry and Immunocytochemistry to develop an esophageal minimum essential organoid culture medium (E-MEOM) for culturing murine esophageal organoids (EOs) and establishing an early esophageal squamous cell carcinoma (ESCC) model.