Invitrogen

Villin Monoclonal Antibody (3E5G11)

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抗体检测数据 (6)

Villin Antibody in Immunocytochemistry (ICC/IF)

Villin Antibody in Immunocytochemistry (ICC/IF)

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Villin Antibody (MA5-38658) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of Villin in HeLa cells using Villin Monoclonal Antibody (Product # MA5-38658) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)

Villin Antibody in Immunocytochemistry (ICC/IF)

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Villin Antibody in Western Blot (WB)

Villin Antibody in Western Blot (WB)

Villin Antibody in Western Blot (WB)

Villin Antibody in Flow Cytometry (Flow)

Product Image Gallery

Villin Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis of Villin in HeLa cells using Villin Monoclonal Antibody (Product # MA5-38658) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of Villin in paraffin-embedded cervical cancer tissues using Villin Monoclonal Antibody (Product # MA5-38658) and DAB staining.

Villin Antibody in Western Blot (WB) — Western Blot analysis of Villin using Villin Monoclonal Antibody (Product # MA5-38658) in HEK293 (1) and VIL1 (AA: 1-209)-human IgG-Fc transfected HEK293 (2) cell lysate.

Villin Antibody in Western Blot (WB) — Western Blot analysis using Villin Monoclonal Antibody (Product # MA5-38658) against human VIL1 (AA: 1-209) recombinant protein. (Expected MW is 49.4 kDa)

Villin Antibody in Western Blot (WB) — Western Blot analysis of Villin using Villin Monoclonal Antibody (Product # MA5-38658) in SW620 cell lysate.

Villin Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of Villin in SW620 cells using Villin Monoclonal Antibody (Product # MA5-38658) (green) and negative control (red).

产品信息

MA5-38658

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

-

免疫细胞化学 (ICC/IF)

1:200-1:1,000

-

流式细胞分析 (Flow)

1:200-1:400

-

酶联免疫吸附实验 (ELISA)

1:10,000

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3E5G11

抗原

Purified recombinant fragment of human VIL1 (aa: 1-209) expressed in E. Coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2898570

产品详细信息

This antibody has been tested in indirect ELISA.

靶标信息

Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: OTTHUMP00000207159; Villin-1

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基因别名: D2S1471; VIL; VIL1

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UniProt ID: (Human) P09327

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Entrez Gene ID: (Human) 7429

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calcium ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding lysophosphatidic acid binding identical protein binding protein homodimerization activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process actin filament binding non-motor actin binding protein

intestinal D-glucose absorption protein complex assembly apoptotic process epidermal growth factor receptor signaling pathway regulation of cell shape response to bacterium positive regulation of epithelial cell migration actin filament polymerization actin filament depolymerization positive regulation of cell migration positive regulation of actin filament depolymerization epithelial cell differentiation positive regulation of actin filament bundle assembly regulation of microvillus length cellular response to hepatocyte growth factor stimulus positive regulation of multicellular organism growth negative regulation of cysteine-type endopeptidase activity involved in apoptotic process actin nucleation actin filament severing barbed-end actin filament capping regulation of actin nucleation actin filament capping cytoplasmic actin-based contraction involved in cell motility regulation of wound healing cellular response to epidermal growth factor stimulus positive regulation of establishment of protein localization to plasma membrane terminal web assembly regulation of lamellipodium morphogenesis positive regulation of lamellipodium morphogenesis