Invitrogen

Villin Recombinant Rabbit Monoclonal Antibody (JU34-75)

查看其他41个Villin抗体

抗体检测数据 (8)

Villin Antibody in Immunocytochemistry (ICC/IF)

Villin Antibody in Immunocytochemistry (ICC/IF)

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Villin Antibody (MA5-34620) in ICC/IF

Immunofluorescent analysis of villin1 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with villin1 monoclonal antibody (Product # MA5-34620), followed by DAPI (blue).

Villin Antibody in Immunocytochemistry (ICC/IF)

Villin Antibody in Immunocytochemistry (ICC/IF)

Villin Antibody in Immunocytochemistry (ICC/IF)

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Villin Antibody in Western Blot (WB)

Villin Antibody in Flow Cytometry (Flow)

Product Image Gallery

Villin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of villin1 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with villin1 monoclonal antibody (Product # MA5-34620), followed by DAPI (blue).

Villin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of villin1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with villin1 monoclonal antibody (Product # MA5-34620), followed by DAPI (blue).

Villin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of villin1 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with villin1 monoclonal antibody (Product # MA5-34620), followed by DAPI (blue).

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of villin1 in paraffin-embedded human colon cancer tissue. Samples were incubated with villin1 monoclonal antibody (Product # MA5-34620), and followed by hematoxylin.

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of villin1 in paraffin-embedded human kidney tissue. Samples were incubated with villin1 monoclonal antibody (Product # MA5-34620), and followed by hematoxylin.

Villin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of villin1 in paraffin-embedded mouse colon tissue. Samples were incubated with villin1 monoclonal antibody (Product # MA5-34620), and followed by hematoxylin.

Villin Antibody in Western Blot (WB) — Western blot analysis of villin1 in Positive control: Lane 1: Mouse colon, Lane 2: Human small intestine, Lane 3: Human colon, Lane 4: Rat kidney. Samples were incubated with villin1 monoclonal antibody (Product # MA5-34620), at a dilution of 1:500.

Villin Antibody in Flow Cytometry (Flow) — Flow cytometry of villin1 in HeLa cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with villin1 monoclonal antibody (Product # MA5-34620) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.

产品信息

MA5-34620

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:500-1:2,000

-

流式细胞分析 (Flow)

1:50-1:100

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JU34-75

抗原

Synthetic peptide within Human villin1 aa 176-225

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Wet ice

AB_2848528

产品详细信息

Positive Control: Mouse colon tissue lysate, human small intestine tissue lysate, human colon tissue lysate, rat kidney tissue lysate, Hela, HepG2, LOVO, human colon cancer tissue, human kidney, mouse colon tissue.

靶标信息

Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: OTTHUMP00000207159; Villin-1

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基因别名: D2S1471; VIL; VIL1

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UniProt ID: (Human) P09327

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Entrez Gene ID: (Human) 7429

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calcium ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding lysophosphatidic acid binding identical protein binding protein homodimerization activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process actin filament binding non-motor actin binding protein

intestinal D-glucose absorption protein complex assembly apoptotic process epidermal growth factor receptor signaling pathway regulation of cell shape response to bacterium positive regulation of epithelial cell migration actin filament polymerization actin filament depolymerization positive regulation of cell migration positive regulation of actin filament depolymerization epithelial cell differentiation positive regulation of actin filament bundle assembly regulation of microvillus length cellular response to hepatocyte growth factor stimulus positive regulation of multicellular organism growth negative regulation of cysteine-type endopeptidase activity involved in apoptotic process actin nucleation actin filament severing barbed-end actin filament capping regulation of actin nucleation actin filament capping cytoplasmic actin-based contraction involved in cell motility regulation of wound healing cellular response to epidermal growth factor stimulus positive regulation of establishment of protein localization to plasma membrane terminal web assembly regulation of lamellipodium morphogenesis positive regulation of lamellipodium morphogenesis