Invitrogen

Vimentin Polyclonal Antibody

Vimentin Antibody in Immunocytochemistry (ICC/IF)

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Vimentin Antibody (PA1-16759) in ICC/IF

Immunofluorescence analysis of Vimentin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vimentin Chicken Polyclonal Antibody (Product # PA1-16759) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Chicken IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More

Vimentin Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-16759

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10,000-1:20,000

免疫组化 (IHC)

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

1:150

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

1:200-1:5000

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Bovine, Dog, Chicken, Horse, Human, Mouse, Pig, Rat

已发表种属

Human, Mouse

宿主/亚型

Chicken / IgY

分类

Polyclonal

类型

Antibody

抗原

The immunogen used to generate our antibody was recombinant vimentin purified from E. coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

16.6 mg/mL

纯化类型

IgY fraction

内含物

0.035% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2257294

靶标信息

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Systematic estimation of biological age of in vitro cell culture systems by an age-associated marker panel.

Frontiers in aging

Hartmann C,Herling L,Hartmann A,Köckritz V,Fuellen G,Walter M,Hermann A

PA1-16759 was used in Immunohistochemistry-immunofluorescence to suggest an easy-to-use biomarker-based panel of classical age markers to estimate the biological age of cell culture systems that can be used in standard cell culture laboratories.

Tue Mar 07 00:00:00 UTC 2023

Patient-derived organoids identify an apico-basolateral polarity switch associated with survival in colorectal cancer.

Journal of cell science

Canet-Jourdan C,Pagès DL,Nguyen-Vigouroux C,Cartry J,Zajac O,Desterke C,Lopez JB,Gutierrez-Mateyron E,Signolle N,Adam J,Raingeaud J,Polrot M,Gonin P,Mathieu JRR,Souquere S,Pierron G,Gelli M,Dartigues P,Ducreux M,Barresi V,Jaulin F

PA1-16759 was used in Immunocytochemistry to study the architecture, invasive behavior and chemoresistance of tumor cell intermediates.

Fri Jul 15 00:00:00 UTC 2022

RhoA drives actin compaction to restrict axon regeneration and astrocyte reactivity after CNS injury.

Neuron

Stern S,Hilton BJ,Burnside ER,Dupraz S,Handley EE,Gonyer JM,Brakebusch C,Bradke F

PA1-16759 was used in Immunohistochemistry to demonstrate how extracellular inhibitors regulate axon regeneration, shed light on the capacity of reactive astrocytes to be growth inhibitory after CNS injury, and reveal cell-specific RhoA targeting as a promising therapeutic avenue.

Wed Nov 03 00:00:00 UTC 2021

ROCK2 inhibition triggers the collective invasion of colorectal adenocarcinomas.

The EMBO journal

Libanje F,Raingeaud J,Luan R,Thomas Z,Zajac O,Veiga J,Marisa L,Adam J,Boige V,Malka D,Goéré D,Hall A,Soazec JY,Prall F,Gelli M,Dartigues P,Jaulin F

PA1-16759 was used in Immunohistochemistry-immunofluorescence to identify FARP2 as the Rac1 GEF necessary for CRC collective invasion.

Mon Jul 15 00:00:00 UTC 2019

Biomimetic electromechanical stimulation to maintain adult myocardial slices in vitro.

Nature communications

Watson SA,Duff J,Bardi I,Zabielska M,Atanur SS,Jabbour RJ,Simon A,Tomas A,Smolenski RT,Harding SE,Perbellini F,Terracciano CM

PA1-16759 was used in Immunohistochemistry-immunofluorescence to show biomimetic electromechanical stimulation of adult cardiac tissue can maintain structural, functional and transcriptional properties.

Wed May 15 00:00:00 UTC 2019

4-methylumbelliferone Prevents Liver Fibrosis by Affecting Hyaluronan Deposition, FSTL1 Expression and Cell Localization.

International journal of molecular sciences

Andreichenko IN,Tsitrina AA,Fokin AV,Gabdulkhakova AI,Maltsev DI,Perelman GS,Bulgakova EV,Kulikov AM,Mikaelyan AS,Kotelevtsev YV

PA1-16759 was used in Immunohistochemistry to provide insight in the alleviation of CCl4 induced liver fibrosis by 4-methylumbelliferone through the reduction of hyaluronan deposition, dowregulation of FSTL1 expression and suppression of hepatic stellate cell trans-differentiation and altered macrophage localisation.