Invitrogen

Vimentin Monoclonal Antibody (V9), eFluor™ 660, eBioscience™

Vimentin Antibody in Immunocytochemistry (ICC/IF)

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Vimentin Antibody (50-9897-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized C6 cells stained with 5 µg/mL of Mouse IgG1 Isotype Control eFluor® 660 (left) or 5 µg/mL of Anti-Vimentin eFluor® 660 (right). Nuclei are stained with DAPI.

Vimentin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Vimentin Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

50-9897-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-Dependent

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

5 µg/mL

查看6篇已发表文章 6篇已发表文章

流式细胞分析 (Flow)

- -

产品规格

种属反应

Dog, Chicken, Human, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574410

产品详细信息

Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils.

This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin.

Applications Reported: This V9 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This V9 antibody has been tested by immunocytochemistry of fixed and permeabilized cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: epididymis luminal protein 113; FLJ36605; RP11-124N14.1; VIME; Vimentin; vimentin C-terminus

查看更多收起

基因别名: CTRCT30; HEL113; VIM

查看更多收起

UniProt ID: (Human) P08670, (Dog) A0A8C0N8E3, (Rat) P31000

查看更多收起

Entrez Gene ID: (Chicken) 420519, (Human) 7431, (Dog) 477991, (Rat) 81818

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功能

glycoprotein binding double-stranded RNA binding structural constituent of cytoskeleton structural constituent of eye lens protein binding protein C-terminus binding identical protein binding scaffold protein binding keratin filament binding structural molecule activity kinase binding protein kinase binding protein phosphatase 2A binding

参与通路

movement of cell or subcellular component positive regulation of gene expression negative regulation of neuron projection development astrocyte development viral process muscle filament sliding intermediate filament organization Bergmann glial cell differentiation SMAD protein signal transduction lens fiber cell development aging response to radiation response to zinc ion response to selenium ion response to estradiol response to vitamin D skeletal muscle tissue regeneration cellular response to fibroblast growth factor stimulus intermediate filament-based process fat cell differentiation decidualization regulation of axonogenesis positive regulation of glial cell proliferation regulation of Schwann cell migration

Vimentin Monoclonal Antibody (V9), eFluor™ 660, eBioscience™

Vimentin Antibody (50-9897-82) in ICC/IF

产品信息

50-9897-82

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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