NeoBiotechnologies

Vimentin (Mesenchymal Cell Marker) Recombinant Mouse Monoclonal Antibody (rVIM/6914)

Vimentin (Mesenchymal Cell Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 3

Vimentin (Mesenchymal Cell Marker) Antibody (7431-MSM14-P1) in IHC (P)

Formalin-fixed, paraffin-embedded human tonsil stained with Vimentin Mouse Recombinant Monoclonal Antibody (rVIM/6914). Inset: PBS instead of primary, secondary antibody control.

Vimentin (Mesenchymal Cell Marker) Antibody in Western Blot (WB)

Vimentin (Mesenchymal Cell Marker) Antibody in Immunoelectrophoresis (IE)

Product Image Gallery

产品信息

7431-MSM14-P1

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

免疫电泳 (IE)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

rVIM/6914

抗原

Recombinant fragment (around aa366-466) of human Vimentin protein.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

200 µg/mL

纯化类型

Protein A/G

保存液

PBS with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody is stable for 24 months.

Positive Control: Human sarcomas ormelanomas. Cellular Localization: Cytoplasm

Specificity Comments: This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

靶标信息

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。