Invitrogen

Vinculin Monoclonal Antibody (7F9), eBioscience™

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Vinculin Antibody (14-9777-82) in WB

Knockout of Vinculin was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR948199_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Vinculin was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa Vinculin KO (Lane 3) . The samples were electrophoresed using . Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed wi... View More

Vinculin Antibody in Immunocytochemistry (ICC/IF)

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产品信息

14-9777-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

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免疫组化 (IHC)

- -

免疫组化（石蜡） (IHC (P))

Assay-Dependent

免疫组化（冰冻） (IHC (F))

5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

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in situ PLA (PLA)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

7F9

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2573028

产品详细信息

Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis.

Applications Reported: This 7F9 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections and formalin-fixed paraffin embedded tissue sections, immunocytochemistry, and microscopy.

Applications Tested: This 7F9 antibody has been tested by immunocytochemistry on methanol-fixed or formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 5 µg/mL. This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval buffer and can be used at less than or equal to 5 µg/mL. The antibody has been tested by western blot on reduced and non-reduced cell lysates and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Structure-based Design Approach for Generating High Affinity BRD4 D1-Selective Chemical Probes.

Journal of medicinal chemistry

Cui H,Divakaran A,Hoell ZJ,Ellingson MO,Scholtz CR,Zahid H,Johnson JA,Griffith EC,Gee CT,Lee AL,Khanal S,Shi K,Aihara H,Shah VH,Lee RE,Harki DA,Pomerantz WCK

14-9777-82 was used in Western Blotting to provide new design rules for selective inhibitors of an individual BET bromodomain.

Thu Feb 10 00:00:00 UTC 2022

Fetal Programming by Methyl Donor Deficiency Produces Pathological Remodeling of the Ascending Aorta.

Arteriosclerosis, thrombosis, and vascular biology

Balint B,Hergalant S,Camadro JM,Blaise S,Vanalderwiert L,Lignières L,Guéant-Rodriguez RM,Guéant JL

14-9777-82 was used in Proximity Ligation Assay to conclude that MDD fetal programming produced altered integrity and remodeling of ascending aorta during aging and irreversible MARS-associated homocysteinylation of key proteins of extracellular matrix and elastin homeostasis.

Tue Jun 01 00:00:00 UTC 2021

Miro1-mediated mitochondrial positioning supports subcellular redox status.

Redox biology

Alshaabi H,Shannon N,Gravelle R,Milczarek S,Messier T,Cunniff B

14-9777 was used in Western Blotting to report that deletion of Miro1 significantly restricts subcellular H2O2 levels to the perinuclear space which directly affects intracellular responses to elevated mitochondrial ROS.

Fri Jan 01 00:00:00 UTC 2021

Miro1-mediated mitochondrial positioning supports subcellular redox status.

Redox biology

Alshaabi H,Shannon N,Gravelle R,Milczarek S,Messier T,Cunniff B

Fri Jan 01 00:00:00 UTC 2021

Inhibiting IRE1α-endonuclease activity decreases tumor burden in a mouse model for hepatocellular carcinoma.

eLife

Pavlović N,Calitz C,Thanapirom K,Mazza G,Rombouts K,Gerwins P,Heindryckx F

14-9777 was used in Western Blotting to study the role of endoplasmic reticulum stress in the cross-talk between stellate and hepatocellular carcinoma cells.

Mon Oct 26 00:00:00 UTC 2020

Inhibiting IRE1α-endonuclease activity decreases tumor burden in a mouse model for hepatocellular carcinoma.

eLife

Pavlović N,Calitz C,Thanapirom K,Mazza G,Rombouts K,Gerwins P,Heindryckx F

14-9777 was used in Western Blotting to study the role of endoplasmic reticulum stress in the cross-talk between stellate and hepatocellular carcinoma cells.

Mon Oct 26 00:00:00 UTC 2020

Substrate properties modulate cell membrane roughness by way of actin filaments.

Scientific reports

Chang CH,Lee HH,Lee CH

Published figure using Vinculin monoclonal antibody (Product # 14-9777-82) in Immunocytochemistry

Tue Aug 22 00:00:00 UTC 2017

Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins.

Cell reports

Davis EM,Kim J,Menasche BL,Sheppard J,Liu X,Tan AC,Shen J

14-9777 was used in Western Blotting to characterise the biogenesis of GPI-anchored proteins.

Tue Jun 23 00:00:00 UTC 2015

生物信息学

蛋白别名: epididymis luminal protein 114; Metavinculin; metavinculin {ECO:0000250|UniProtKB:P18206}; MV; RP11-178G16.3; vcl {ECO:0000250|UniProtKB:P18206}; vinc; Vinculin; vinculin {ECO:0000250|UniProtKB:P18206}

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基因别名: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; HEL114; MV; MVCL; VCL

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UniProt ID: (Human) P18206, (Mouse) Q64727, (Rat) P85972

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Entrez Gene ID: (Human) 7414, (Mouse) 22330, (Rat) 305679

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