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WDYHV1 Antibody (PA5-78518) in ICC/IF
WDYHV1 Polyclonal Antibody detects WDYHV1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WDYHV1 protein stained by WDYHV1 Polyclonal Antibody (Product # PA5-78518) diluted at 1:200. Blue: Hoechst 33342 staining.
产品信息
PA5-78518
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human WDYHV1. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.66 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 0.1M glycine, 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2736795
产品详细信息
Predicted Reactivity: Rat (88%), Rhesus Monkey (97%), Bovine (83%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: N-terminal Gln amidase; nt(Q)-amidase; Protein N-terminal glutamine amidohydrolase; Protein NH2-terminal glutamine deamidase; WDYHV motif-containing protein 1
基因别名: 2410187C16Rik; AU014961; AW550036; C8orf32; NTAQ1; WDYHV1
UniProt ID: (Human) Q96HA8, (Mouse) Q80WB5
Entrez Gene ID: (Human) 55093, (Mouse) 76773
protein binding
protein-N-terminal glutamine amidohydrolase activity
histone deacetylase activity
protein-N-terminal asparagine amidohydrolase activity
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
iprodione amidohydrolase activity
(3,5-dichlorophenylurea)acetate amidohydrolase activity
4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity
didemethylisoproturon amidohydrolase activity
N-isopropylacetanilide amidohydrolase activity
N-cyclohexylformamide amidohydrolase activity
isonicotinic acid hydrazide hydrolase activity
cis-aconitamide amidase activity
gamma-N-formylaminovinylacetate hydrolase activity
N2-acetyl-L-lysine deacetylase activity
O-succinylbenzoate synthase activity
indoleacetamide hydrolase activity
N-acetylcitrulline deacetylase activity
N-acetylgalactosamine-6-phosphate deacetylase activity
diacetylchitobiose deacetylase activity
chitooligosaccharide deacetylase activity
