Invitrogen

WEE1 Polyclonal Antibody

WEE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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WEE1 Antibody (PA5-15385) in IHC (P)

Immunohistochemistry analysis of WEE1 in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with WEE1 polyclonal antibody (Product # PA5-15385) which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepato... View More

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产品信息

PA5-15385

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 144-173 amino acids from the central region of human WEE1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Ammonium sulfate precipitation, Size-exclusion - Dialysis

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2257246

靶标信息

WEE1, which is classified as a Ser/Thr kinase by sequence analysis, has been shown to inactivate multiple CDKs by tyrosine phosphorylation. Wee1 protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。