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WIPI2 Antibody (PA5-78465) in ICC/IF
Immunofluorescence analysis of WIPI2 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with WIPI2 Polyclonal Antibody (Product # PA5-78465) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a... View More
产品信息
PA5-78465
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human WIPI2. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.57 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2736796
产品详细信息
Positive Control: K562, THP-1, Jurkat
Predicted Reactivity: Mouse (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: WD repeat domain phosphoinositide-interacting protein 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI-2; WIPI49-like protein 2
基因别名: ATG18B; Atg21; CGI-50; WIPI-2; WIPI2
UniProt ID: (Human) Q9Y4P8, (Rat) Q6AY57
Entrez Gene ID: (Human) 26100, (Rat) 288498
