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WISP2 Antibody (500-P212-1MG) in WB
Western Blot: To detect Human CTGFL/WISP-2 by Western Blot analysis WISP2 Polyclonal Antibody (Product # 500-P212-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGFL/WISP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P212-1MG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human CTGFL/WISP-2
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929838
产品详细信息
AA Sequence of recombinant protein: MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CTGFL/WISP-2. Anti-Human CTGFL/WISP-2-specific antibody was purified by affinity chromatography employing an immobilized Human CTGFL/WISP-2 matrix.
Sandwich ELISA: To detect Human CTGFL/WISP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CTGFL/WISP-2 (500-P212Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CTGFL/WISP-2.
Western Blot: To detect Human CTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGFL/WISP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Wisp2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. Wisp2 lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58; CTGF-L; WISP-2; WNT1-inducible-signaling pathway protein 2
基因别名: CCN5; CT58; CTGF-L; CTGFL; UNQ228/PRO261; WISP2
UniProt ID: (Human) O76076
Entrez Gene ID: (Human) 8839
