Invitrogen

WNT1 Polyclonal Antibody

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WNT1 Antibody (PA5-34776) in WB

WNT1 Polyclonal Antibody detects Wnt1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human WNT1-transfected 293T cells. 10% SDS PAGE. WNT1 Polyclonal Antibody (Product # PA5-34776) dilution: 1:1,000.

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产品信息

PA5-34776

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 234 of WNT1 (Uniprot ID#P04628)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2552128

产品详细信息

Recommended positive controls: 30 µg 293T whole cell lysate/extract, 30 µg whole cell lysate/extract of human WNT1-transfected 293T cells.

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (81%), Bovine (99%).

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Proto-oncogene Int-1 homolog; Proto-oncogene Wnt-1; wingless-related MMTV integration site 1; Wingless-type MMTV integration site 1 homolog; Wingless-type MMTV integration site 1, homolog; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)

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基因别名: BMND16; INT1; OI15; WNT1

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UniProt ID: (Human) P04628

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Entrez Gene ID: (Human) 7471, (Rat) 24881

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功能

frizzled binding cytokine activity morphogen activity protein domain specific binding transcription regulatory region DNA binding receptor agonist activity molecular_function intercellular signal molecule

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