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WNT3A Antibody (500-P251BT-25UG) in ELISA
Sandwich ELISA: To detect hWNT-3a by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of WNT3A Polyclonal Antibody, Biotin (Product # 500-P251BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech WNT3A Polyclonal Antibody (Product # 500-P251-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hWNT-3a.
产品信息
500-P251BT-25UG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human Wnt-3a
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930070
产品详细信息
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Wnt-3a. Anti-Human Wnt-3a-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hWNT-3a by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human WNT-3a (500-P251) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hWNT-3a.
Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hWNT-3a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P251BT-1MG will be provided as 2 x 500 µg
靶标信息
Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.
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生物信息学
蛋白别名: MGC119418; MGC119419; MGC119420; Protein Wnt-3a; wingless-type MMTV integration site family member 3A; wingless-type MMTV integration site family, member 3A; Wnt 3a; WNT3A protein
基因别名: WNT3A
UniProt ID: (Human) P56704
Entrez Gene ID: (Human) 89780
osteoblast differentiation
in utero embryonic development
positive regulation of cytokine production
positive regulation of protein phosphorylation
heart looping
positive regulation of receptor internalization
negative regulation of heart induction by canonical Wnt signaling pathway
axon guidance
synapse assembly
positive regulation of cell proliferation
COP9 signalosome assembly
negative regulation of neuron projection development
Wnt signaling pathway
spinal cord association neuron differentiation
hippocampus development
cell proliferation in forebrain
Wnt signaling pathway involved in forebrain neuroblast division
dorsal/ventral neural tube patterning
hemopoiesis
neuron differentiation
extracellular matrix organization
mammary gland development
positive regulation of B cell proliferation
positive regulation of protein binding
positive regulation of peptidyl-serine phosphorylation
cell proliferation in midbrain
cellular protein localization
positive regulation of catenin import into nucleus
skeletal muscle cell differentiation
post-anal tail morphogenesis
synaptic vesicle recycling
inner ear morphogenesis
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of mesodermal cell fate specification
paraxial mesodermal cell fate commitment
positive regulation of skeletal muscle tissue development
positive regulation of collateral sprouting in absence of injury
negative regulation of axon extension involved in axon guidance
negative regulation of neurogenesis
positive regulation of sequence-specific DNA binding transcription factor activity
palate development
canonical Wnt signaling pathway
positive regulation of protein tyrosine kinase activity
positive regulation of dermatome development
canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment
regulation of microtubule cytoskeleton organization
platelet aggregation
cellular response to retinoic acid
axis elongation involved in somitogenesis
positive regulation of canonical Wnt signaling pathway
positive regulation of protein localization to plasma membrane
negative regulation of dopaminergic neuron differentiation
beta-catenin destruction complex disassembly
Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation
positive regulation of cell-cell adhesion mediated by cadherin
positive regulation of canonical Wnt signaling pathway involved in controlling type B pancreatic cell proliferation
positive regulation of neural precursor cell proliferation
positive regulation of hepatocyte proliferation
positive regulation of transcription regulatory region DNA binding
positive regulation of cardiac muscle cell differentiation
