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XPD Antibody (PA5-120282) in WB
Western blot analysis of XPD in extracts of various cell lines. Samples were incubated with XPD Polyclonal antibody (Product # PA5-120282) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
产品信息
PA5-120282
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human XPD/XPD/ERCC2 (NP_0011243391)
偶联物
形式
Liquid
浓度
0.69 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.05% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2913854
产品详细信息
Positive test controls include: HeLa, K-562, 293T, MCF7, mouse lung, mouse testis, rat testis. The target is usually found in the following locations: Cytoplasm, Nucleus, cytoskeleton, spindle.
Immunogen sequence: MRELKRTLDA KGHGVLEMPS GTGKTVSLLA LIMAYQRAYP LEVTKLIYCS RTVPEIEKVI EELRKLLNFY EKQEGEKLPF LGLALSSRKN LCIHPEVTPL RFGKDVDGKC HSLTASYVRA QYQHDTSLPH CRFYEEFDAH GREVPLPAGI YNLDDLKALG RRQGWCPYFL ARYSILHANV
靶标信息
XPD is involved with the nucleotide excision repair pathway that is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Basic transcription factor 2 80 kDa subunit; BTF2 p80; CXPD; DNA 5'-3' helicase XPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; excision repair cross-complementation group 2; excision repair cross-complementing rodent repair deficiency, complementation group 2; General transcription and DNA repair factor IIH helicase subunit XPD; MGC102762; MGC126218; MGC126219; TFIIH 80 kDa subunit; TFIIH basal transcription factor complex 80 kDa subunit; TFIIH basal transcription factor complex helicase subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH basal transcription factor complex helicase XPD subunit; TFIIH p80; TFIIH subunit XPD; xeroderma pigmentosum complementary group D; Xeroderma pigmentosum group D-complementing protein
基因别名: AA407812; AU020867; AW240756; COFS2; CXPD; EM9; Ercc-2; ERCC2; TFIIH; TTD; TTD1; XPD; XPDC
UniProt ID: (Human) P18074, (Mouse) O08811
Entrez Gene ID: (Human) 2068, (Mouse) 13871, (Rat) 308415
DNA binding
ATP-dependent DNA helicase activity
protein kinase activity
protein binding
ATP binding
protein C-terminus binding
DNA-dependent ATPase activity
RNA polymerase II carboxy-terminal domain kinase activity
5'-3' DNA helicase activity
metal ion binding
protein N-terminus binding
4 iron, 4 sulfur cluster binding
nucleotide binding
nucleic acid binding
helicase activity
ATP-dependent helicase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
iron-sulfur cluster binding
molecular_function
DNA helicase
nucleotide-excision repair, DNA duplex unwinding
response to hypoxia
in utero embryonic development
transcription-coupled nucleotide-excision repair
nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
transcription initiation from RNA polymerase I promoter
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
7-methylguanosine mRNA capping
protein phosphorylation
apoptotic process
response to oxidative stress
chromosome segregation
aging
cell proliferation
UV protection
post-embryonic development
viral process
spinal cord development
extracellular matrix organization
bone mineralization
central nervous system myelin formation
nucleotide-excision repair, DNA incision
multicellular organism growth
hair cell differentiation
embryonic cleavage
erythrocyte maturation
positive regulation of DNA binding
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
hair follicle maturation
hematopoietic stem cell differentiation
global genome nucleotide-excision repair
regulation of mitotic cell cycle phase transition
nucleobase-containing compound metabolic process
DNA repair
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
response to UV
hair cycle process
DNA duplex unwinding
skin development
