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XRCC1 Antibody (PA5-80237) in ICC/IF
Immunocytochemistry analysis of XRCC1 using anti-XRCC1 antibody (Product # PA5-80237) . XRCC1 was detected in a section of NIH3T3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-XRCC1 antibody (Product # PA5-80237) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. ... View More
Product Details
PA5-80237
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human XRCC1 recombinant protein (Position: E538-A633).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2747351
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human 293T whole cell, human K562 whole cell, human Jurkat whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue. IHC: rat kidney tissue, human placenta tissue, human colon cancer tissue, human mammary cancer tissue IHC-F: rat intestine tissue, human placenta tissue, mouse intestine tissue. ICC/IF: NIH3T3 cell. Flow: THP-1 cell.
Target Information
XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70 kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA ligase III-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA repair protein XRCC1; X-ray repair complementing defective repair in Chinese hamster cells 1; x-ray repair cross-complementing group 1 protein; X-ray repair cross-complementing protein 1
Gene Aliases: RCC; Xrcc-1; XRCC1
UniProt ID: (Human) P18887, (Rat) Q9ESZ0, (Mouse) Q60596
Entrez Gene ID: (Human) 7515, (Rat) 84495, (Mouse) 22594
single strand break repair
response to hypoxia
DNA repair
base-excision repair
cellular response to DNA damage stimulus
response to organic substance
hippocampus development
response to drug
double-strand break repair via homologous recombination
transcription-coupled nucleotide-excision repair
base-excision repair, DNA ligation
nucleotide-excision repair, DNA gap filling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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