Invitrogen

YARS Polyclonal Antibody

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YARS Antibody (PA5-121039) in WB

Western blot analysis of YARS in extracts of various cell lines. Samples were incubated with YARS Polyclonal antibody (Product # PA5-121039) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

YARS Antibody in Immunoprecipitation (IP)

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产品信息

PA5-121039

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

免疫沉淀 (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human YARS (NP_003671.1).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.49 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2914611

产品详细信息

Positive test controls include: HeLa, SKOV3, Mouse brain, Mouse skeletal muscle, Rat brain. The target is usually found in the following locations: Cytoplasm.

Immunogen sequence: MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKIYW GTATTGKPHV AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVSYYENV IKAMLESIGV PLEKLKFIKG TDYQLSKEYT LDVYRLSSVV TQHDSKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGIDQRK IFTFAEKYLP ALGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC EPGNVENNGV LSFIKHVLFP LKSEFVILRD EKWGGNKTYT AYVDLEKDFA

靶标信息

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: tyrosine tRNA ligase 1, cytoplasmic; Tyrosine--tRNA ligase, cytoplasmic; tyrosyl trna synthetase; tyrosyl--tRNA ligase; Tyrosyl-tRNA synthetase; tyrosyl-tRNA synthetase, cytoplasmic; TyrRS

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基因别名: AL024047; AU018965; CMTDIC; TYRRS; YARS; YARS1; YRS; YTS

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UniProt ID: (Human) P54577, (Mouse) Q91WQ3, (Rat) Q4KM49

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Entrez Gene ID: (Human) 8565, (Mouse) 107271, (Rat) 313047

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