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YBX1 Antibody (711277) in ICC/IF
For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized for detection of endogenous YB1 using Anti-YB1 Recombinant Rabbit Superclonal™ Antibody (Product # 711277, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of YB1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). ... View More
产品信息
711277
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
10HCLC
抗原
Peptides corresponding to human YB1 (aa174-190, 251-270)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2663065
产品详细信息
This antibody is predicted to react with Monkey, Bovine, Mouse, Rat
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BP8; CBF-A; CCAAT-binding; CCAAT-binding transcription factor I subunit A; DBPB; DNA binding protein B; DNA-binding protein B; EFI-A; Enhancer factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; MDR NF1; MGC104858; MGC110976; MGC117250; nuclease sensitive element binding protein 1; Nuclease-sensitive element-binding protein 1; Y box binding protein 1; Y-box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YB-1
基因别名: 1700102N10Rik; BP-8; C79409; CBF-A; CSDA2; CSDB; DBPB; EF1A; EFI-A; MDR-NF1; Msy-1; MSY1; mYB-1a; NSEP-1; NSEP1; YB-1; YB1; YBX1
UniProt ID: (Human) P67809, (Mouse) P62960
Entrez Gene ID: (Human) 4904, (Mouse) 22608
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
double-stranded DNA binding
single-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
RNA binding
protein binding
poly(A) RNA binding
GTPase binding
p53 binding
nucleic acid binding
chromatin binding
mRNA binding
transcription factor binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
mRNA processing
positive regulation of cell proliferation
RNA splicing
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin receptor signaling pathway
negative regulation of striated muscle cell differentiation
positive regulation of cell division
CRD-mediated mRNA stabilization
mRNA splicing, via spliceosome
protein localization to cytoplasmic stress granule
