Bethyl Laboratories

YKT6 Polyclonal Antibody

查看其他9个YKT6抗体

抗体检测数据 (2)

YKT6 Antibody in Western Blot (WB)

YKT6 Antibody in Western Blot (WB)

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YKT6 Antibody (A305-479A-T) in WB

Detection of human and mouse YKT6 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-YKT6 antibody (Product # A305-479A) (lot A305-479A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

YKT6 Antibody in Western Blot (WB)

YKT6 Antibody in Immunoprecipitation (IP)

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YKT6 Antibody in Western Blot (WB) — Detection of human and mouse YKT6 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-YKT6 antibody (Product # A305-479A) (lot A305-479A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

YKT6 Antibody in Immunoprecipitation (IP) — Detection of human YKT6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-YKT6 antibody (Product # A305-479A) (lot 1) used for IP at 6 µg per reaction. YKT6 was also immunoprecipitated by rabbit anti-YKT6 antibody A305-480A. For blotting immunoprecipitated YKT6, A305-479A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

产品信息

A305-479A-T

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,500

-

免疫沉淀 (IP)

2-10 µg/mg lysate

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 125 to 175 of Human Synaptobrevin homolog YKT6.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

phosphate/tris citrate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Bovine

靶标信息

This gene product is one of the SNARE recognition molecules implicated in vesicular transport between secretory compartments. It is a membrane associated, isoprenylated protein that functions at the endoplasmic reticulum-Golgi transport step. This protein is highly conserved from yeast to human and can functionally complement the loss of the yeast homolog in the yeast secretory pathway.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: prenylated SNARE protein (Ykt6); R-SNARE; SNARE protein Ykt6; Synaptobrevin homolog YKT6; YKT6 v-SNARE protein; YKT6, S. cerevisiae, homolog of

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基因别名: 0610042I15Rik; 1810013M05Rik; AW105923; YKT6

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UniProt ID: (Human) O15498, (Mouse) Q9CQW1

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Entrez Gene ID: (Human) 10652, (Mouse) 56418

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SNARE binding SNAP receptor activity protein-cysteine S-palmitoyltransferase activity cadherin binding involved in cell-cell adhesion dihydrolipoamide branched chain acyltransferase activity sterol O-acyltransferase activity N-acetyltransferase activity O-acyltransferase activity palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity carnitine O-acyltransferase activity acetyltransferase activity C-acyltransferase activity palmitoyltransferase activity N-acyltransferase activity acylglycerol O-acyltransferase activity serine O-acyltransferase activity O-acetyltransferase activity O-octanoyltransferase activity octanoyltransferase activity O-palmitoyltransferase activity S-acyltransferase activity S-acetyltransferase activity S-malonyltransferase activity malonyltransferase activity C-palmitoyltransferase activity transferase activity succinyltransferase activity N-succinyltransferase activity O-succinyltransferase activity S-succinyltransferase activity sinapoyltransferase activity O-sinapoyltransferase activity peptidyl-lysine N6-myristoyltransferase activity peptidyl-lysine N6-palmitoyltransferase activity benzoyl acetate-CoA thiolase activity 3-hydroxybutyryl-CoA thiolase activity 3-ketopimelyl-CoA thiolase activity N-palmitoyltransferase activity myristoyltransferase activity acyl-CoA N-acyltransferase activity protein-cysteine S-myristoyltransferase activity protein-cysteine S-acyltransferase activity dihydrolipoamide S-acyltransferase activity glucosaminyl-phosphotidylinositol O-acyltransferase activity ergosterol O-acyltransferase activity lanosterol O-acyltransferase activity naphthyl-2-oxomethyl-succinyl-CoA succinyl transferase activity 2,4,4-trimethyl-3-oxopentanoyl-CoA 2-C-propanoyl transferase activity 2-methylhexanoyl-CoA C-acetyltransferase activity butyryl-CoA 2-C-propionyltransferase activity 2,6-dimethyl-5-methylene-3-oxo-heptanoyl-CoA C-acetyltransferase activity L-2-aminoadipate N-acetyltransferase activity UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase activity keto acid formate lyase activity Ras palmitoyltransferase activity azetidine-2-carboxylic acid acetyltransferase activity peptidyl-lysine N-acetyltransferase activity, acting on acetyl phosphate as donor acetyl-CoA:L-lysine N6-acetyltransferase

ER to Golgi vesicle-mediated transport vesicle targeting vesicle docking involved in exocytosis vesicle fusion protein transport retrograde transport, endosome to Golgi cell-cell adhesion transport exocytosis metabolic process vesicle-mediated transport membrane fusion