Invitrogen

ZC3HAV1 Polyclonal Antibody

ZC3HAV1 Antibody in Immunocytochemistry (ICC/IF)

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ZC3HAV1 Antibody (PA5-31650) in ICC/IF

ZC3HAV1 Polyclonal Antibody detects ZC3HAV1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZC3HAV1 stained by ZC3HAV1 Polyclonal Antibody (Product # PA5-31650) diluted at 1:1,000. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

ZC3HAV1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ZC3HAV1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

ZC3HAV1 Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-31650

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:20,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 401 and 721 of Human ZC3HAV1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.35 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2549123

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The zinc finger antiviral protein (ZC3HAV1) is a CCCH type zinc finger protein that induces an innate immunity to infections by retrovirus by preventing the accumulation of viral RNAs in the cytoplasm and recruits the RNA processing exosome to degrade target RNAs, thereby inhibiting virus replication. ZC3HAV1 is localized in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner. ZAP is a direct target gene of IRF3 action in cellular antiviral responses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CpG dinucleotide enrichment in the influenza A virus genome as a live attenuated vaccine development strategy.

PLoS pathogens

Sharp CP,Thompson BH,Nash TJ,Diebold O,Pinto RM,Thorley L,Lin YT,Sives S,Wise H,Clohisey Hendry S,Grey F,Vervelde L,Simmonds P,Digard P,Gaunt ER

PA5-31650 was used in Western Blotting to show that ZAP-sensitive CpG enriched viruses that are defective in human systems can yield high titre in vaccine propagation systems, providing a realistic, economically viable platform to augment existing live attenuated vaccines.

Mon May 01 00:00:00 UTC 2023

CpG dinucleotide enrichment in the influenza A virus genome as a live attenuated vaccine development strategy.

PLoS pathogens

Sharp CP,Thompson BH,Nash TJ,Diebold O,Pinto RM,Thorley L,Lin YT,Sives S,Wise H,Clohisey Hendry S,Grey F,Vervelde L,Simmonds P,Digard P,Gaunt ER

Mon May 01 00:00:00 UTC 2023

N(6)-methyladenosine-binding protein YTHDF1 suppresses EBV replication and promotes EBV RNA decay.

EMBO reports

Xia TL,Li X,Wang X,Zhu YJ,Zhang H,Cheng W,Chen ML,Ye Y,Li Y,Zhang A,Dai DL,Zhu QY,Yuan L,Zheng J,Huang H,Chen SQ,Xiao ZW,Wang HB,Roy G,Zhong Q,Lin D,Zeng YX,Wang J,Zhao B,Gewurz BE,Chen J,Zuo Z,Zeng MS

Published figure using ZC3HAV1 polyclonal antibody (Product # PA5-31650) in Immunocytochemistry

Wed Apr 07 00:00:00 UTC 2021

Human cytomegalovirus evades ZAP detection by suppressing CpG dinucleotides in the major immediate early 1 gene.

PLoS pathogens

Lin YT,Chiweshe S,McCormick D,Raper A,Wickenhagen A,DeFillipis V,Gaunt E,Simmonds P,Wilson SJ,Grey F

PA5-31650 was used in Western Blotting to demonstrate that ZAP is a potent host restriction factor against large DNA viruses and that HCMV evades ZAP detection through suppression of CpG dinucleotides within the major immediate early 1 transcript.

Tue Sep 01 00:00:00 UTC 2020

Human cytomegalovirus evades ZAP detection by suppressing CpG dinucleotides in the major immediate early 1 gene.

PLoS pathogens

Lin YT,Chiweshe S,McCormick D,Raper A,Wickenhagen A,DeFillipis V,Gaunt E,Simmonds P,Wilson SJ,Grey F

Tue Sep 01 00:00:00 UTC 2020

Human cytomegalovirus evades ZAP detection by suppressing CpG dinucleotides in the major immediate early 1 gene.

PLoS pathogens

Lin YT,Chiweshe S,McCormick D,Raper A,Wickenhagen A,DeFillipis V,Gaunt E,Simmonds P,Wilson SJ,Grey F

Tue Sep 01 00:00:00 UTC 2020

The Short Form of the Zinc Finger Antiviral Protein Inhibits Influenza A Virus Protein Expression and Is Antagonized by the Virus-Encoded NS1.

Journal of virology

Tang Q,Wang X,Gao G

PA531650 was used in western blot to elucidate the interactions between zinc finger antiviral protein and influenza A virus

Sun Jan 15 00:00:00 UTC 2017

Interferon alpha antagonizes the anti-hepatoma activity of the oncolytic virus M1 by stimulating anti-viral immunity.

Oncotarget

Ying L,Cheng H,Xiong XW,Yuan L,Peng ZH,Wen ZW,Ka LJ,Xiao X,Jing C,Qian TY,Liang GZ,Mei YG,Bo ZW,Liang P

Published figure using ZC3HAV1 polyclonal antibody (Product # PA5-31650) in Immunohistochemistry

Tue Apr 11 00:00:00 UTC 2017

Identification and characterization of alphavirus M1 as a selective oncolytic virus targeting ZAP-defective human cancers.

Proceedings of the National Academy of Sciences of the United States of America

Lin Y,Zhang H,Liang J,Li K,Zhu W,Fu L,Wang F,Zheng X,Shi H,Wu S,Xiao X,Chen L,Tang L,Yan M,Yang X,Tan Y,Qiu P,Huang Y,Yin W,Su X,Hu H,Hu J,Yan G

PA5-31650 was used in western blot to study ZAP-defective human cancers and a characterization of alphavirus M1 as a selective oncolytic virus

Tue Oct 21 00:00:00 UTC 2014

Identification and characterization of alphavirus M1 as a selective oncolytic virus targeting ZAP-defective human cancers.

Proceedings of the National Academy of Sciences of the United States of America

Lin Y,Zhang H,Liang J,Li K,Zhu W,Fu L,Wang F,Zheng X,Shi H,Wu S,Xiao X,Chen L,Tang L,Yan M,Yang X,Tan Y,Qiu P,Huang Y,Yin W,Su X,Hu H,Hu J,Yan G

PA5-31650 was used in western blot to study ZAP-defective human cancers and a characterization of alphavirus M1 as a selective oncolytic virus

Tue Oct 21 00:00:00 UTC 2014

生物信息学

蛋白别名: ADP-ribosyltransferase diphtheria toxin-like 13; ARTD13; Inactive Poly [ADP-ribose] polymerase 13; PARP13; ZAP; Zinc finger antiviral protein; Zinc finger CCCH domain-containing protein 2; Zinc finger CCCH-type antiviral protein 1; zinc finger CCCH-type, antiviral 1

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基因别名: ARTD13; FLB6421; PARP13; PRO1677; ZAP; ZC3H2; ZC3HAV1; ZC3HDC2

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UniProt ID: (Human) Q7Z2W4

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Entrez Gene ID: (Human) 56829

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功能

NAD+ ADP-ribosyltransferase activity protein binding poly(A) RNA binding metal ion binding cadherin binding involved in cell-cell adhesion

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