Invitrogen

ZEB1 Polyclonal Antibody

ZEB1 Antibody in Immunocytochemistry (ICC/IF)

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ZEB1 Antibody (PA5-82982) in ICC/IF

Immunofluorecent analysis of ZEB1 in human cell line U-2 OS using ZEB1 Polyclonal Antibody (Product # PA5-82982). Staining shows localization to nucleoplasm.

ZEB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

ZEB1 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of ZEB1 demonstrates antibody specificity. Immunohistochemical analysis of ZEB1 using anti-ZEB1 Polyclonal Antibody (Product #PA5-82982), shows significant staining of ZEB1 in cerebral cortex and shows minimal or weak staining in liver tissues. The relative expression levels of ZEB1 within each tissue is shown using RNA-Seq. {RE}

产品信息

PA5-82982

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:500-1:1,000

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

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免疫沉淀 (IP)

- -

体外检测 (IV)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human ZEB1. Recombinant protein control fragment (Product #RP-100723).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.3 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2790138

产品详细信息

Immunogen sequence: EAEKPESSVS SATGDGNLSP SQPPLKNLLS LLKAYYALNA QPSAEELSKI ADSVNLPLDV VKKWFEKMQA GQISVQSSEP SSPEPGKVNI PAKNNDQPQS ANANEPQDST VNLQSPLKMT NSPVLPVGST

靶标信息

ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor. It inhibits interleukin-2 (IL-2) gene expression. ZEB1 enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and depending on the cell type. It represses E-cadherin promoters and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 also represses BCL6 transcription in the presence of the corepressor CTBP1. It postively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis. Mutations in the gene can result in corneal dystrophy, posterior polymorphous and corneal dystrophy, Fuchs endothelial 6.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

An atlas of late prenatal human neurodevelopment resolved by single-nucleus transcriptomics.

Nature communications

Ramos SI,Mussa ZM,Falk EN,Pai B,Giotti B,Allette K,Cai P,Dekio F,Sebra R,Beaumont KG,Tsankov AM,Tsankova NM

PA5-82982 was used in Immunocytochemistry, Immunohistochemistry (Paraffin) to present a comprehensive single-nucleus RNA sequencing atlas of over 200,000 nuclei derived from the proliferative germinal matrix and laminating cortical plate of 15 prenatal, non-pathological postmortem samples from 17 to 41 gestational weeks, and 3 adult controls.

Mon Dec 12 00:00:00 UTC 2022

An atlas of late prenatal human neurodevelopment resolved by single-nucleus transcriptomics.

Nature communications

Ramos SI,Mussa ZM,Falk EN,Pai B,Giotti B,Allette K,Cai P,Dekio F,Sebra R,Beaumont KG,Tsankov AM,Tsankova NM

Mon Dec 12 00:00:00 UTC 2022

Phenotypic heterogeneity driven by plasticity of the intermediate EMT state governs disease progression and metastasis in breast cancer.

Science advances

Brown MS,Abdollahi B,Wilkins OM,Lu H,Chakraborty P,Ognjenovic NB,Muller KE,Jolly MK,Christensen BC,Hassanpour S,Pattabiraman DR

PA5-82982 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), In Vitro Assay to develop an advanced multiplexed immunostaining approach using SUM149-derived orthotopic tumors and find that the EMT state and epithelial-mesenchymal heterogeneity are predictive of overall survival in a cohort of stage III breast cancer.

Fri Aug 05 00:00:00 UTC 2022

Phenotypic heterogeneity driven by plasticity of the intermediate EMT state governs disease progression and metastasis in breast cancer.

Science advances

Brown MS,Abdollahi B,Wilkins OM,Lu H,Chakraborty P,Ognjenovic NB,Muller KE,Jolly MK,Christensen BC,Hassanpour S,Pattabiraman DR

Fri Aug 05 00:00:00 UTC 2022

Phenotypic heterogeneity driven by plasticity of the intermediate EMT state governs disease progression and metastasis in breast cancer.

Science advances

Brown MS,Abdollahi B,Wilkins OM,Lu H,Chakraborty P,Ognjenovic NB,Muller KE,Jolly MK,Christensen BC,Hassanpour S,Pattabiraman DR

Fri Aug 05 00:00:00 UTC 2022

Phenotypic heterogeneity driven by plasticity of the intermediate EMT state governs disease progression and metastasis in breast cancer.

Science advances

Brown MS,Abdollahi B,Wilkins OM,Lu H,Chakraborty P,Ognjenovic NB,Muller KE,Jolly MK,Christensen BC,Hassanpour S,Pattabiraman DR

Fri Aug 05 00:00:00 UTC 2022

生物信息学

蛋白别名: delta-crystallin enhancer binding factor 1; DELTA-EF1; MGC133261; Negative regulator of IL2; NIL-2-A; NIL-2-A zinc finger protein; NIL-2A; OTTHUMP00000019405; posterior polymorphous corneal dystrophy 3; RP11-472N13.4; TCF-8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); Zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein

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基因别名: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZEB1; ZFHEP; ZFHX1A

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UniProt ID: (Human) P37275

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Entrez Gene ID: (Human) 6935

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功能