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ZNRF3 Antibody (PA5-120417) in IHC (P)
Immunohistochemistry analysis of ZNRF3 in paraffin-embedded mouse spleen. Samples were incubated with ZNRF3 Polyclonal antibody (Product # PA5-120417) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-120417
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human ZNRF3 (NP_0011939271)
Conjugate
Form
Liquid
Concentration
0.46 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913989
Product Specific Information
Positive test controls include: A-549.
Immunogen sequence: MHPLGLCNNN DEEDLYEYGW VGVVKLEQPE LDPKPCLTVL GKAKRAVQRG ATAVIFDVSE NPEAIDQLNQ GSEDPLKRPV VYVKGADAIK LMNIVNKQKV A
Target Information
The ZNRF3 gene encodes a transmembrane E3 ubiquitin ligase that plays a critical role in regulating the Wnt signaling pathway, which is essential for various biological processes, including embryonic development and cell proliferation. ZNRF3 functions by negatively regulating this pathway through ubiquitination and degradation of Frizzled receptors, thereby suppressing the Wnt signaling. Located on chromosome 22, ZNRF3 acts as a tumor suppressor gene by inhibiting oncogenic Wnt signaling and has been implicated in the formation and progression of several cancers, particularly colorectal cancer. Moreover, ZNRF3 interacts with R-spondin proteins which are known to augment Wnt signaling by targeting ZNRF3 for degradation, thus facilitating cell differentiation and proliferation. Alterations in ZNRF3 expression or mutations can lead to aberrant Wnt signaling, contributing to tumorigenesis. Additionally, ZNRF3's role extends to its participation in lipid metabolism and maintenance of bone homeostasis. It has been studied as a potential therapeutic target due to its central involvement in these regulatory pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTA-292E10.6; E3 ubiquitin-protein ligase ZNRF3; novel C3HC4 type Zinc finger (ring finger); rCG35969-like; RING finger protein 203; RING-type E3 ubiquitin transferase ZNRF3; zinc and ring finger 3; Zinc/RING finger protein 3
Gene Aliases: BK747E2.3; Gm1167; KIAA1133; RNF203; ZNRF3
UniProt ID: (Human) Q9ULT6, (Mouse) Q5SSZ7
Entrez Gene ID: (Human) 84133, (Rat) 685487, (Mouse) 407821
ubiquitin-protein transferase activity
frizzled binding
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase activity
molecular_function
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-dependent protein catabolic process
protein ubiquitination
Wnt receptor catabolic process
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
stem cell proliferation
negative regulation of canonical Wnt signaling pathway
negative regulation of non-canonical Wnt signaling pathway
biological_process
negative regulation of Wnt signaling pathway
Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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