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ZO-1 Antibody (600-401-GU7) in ICC/IF
Immunofluorescence microscopy of Anti-ZO-1 in Caco-2 cells using FITC-conjugated Fluorescent TrueBlot® anti-rabbit IgG (p/n 18-0216-32) for detection. Caco-2 cells were fixed with 4% PFA, blocked (5% mouse serum/0.3% Triton X-100 in 1X PBS) for 1hr, then incubated with 15 µg/mL of anti-ZO-1 primary antibody (Cat. No. 600-401-GU7) at 4°C overnight. Following 3 washes in 1X PBS for 5 min each, 5 µg/mL of FITC-conjugated Fluorescent TrueBlot® anti-rabbit IgG was added and allowed to incubate for 1hr at room temperature. Nuclei were countersta... View More
产品信息
600-401-GU7
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
偶联物
形式
Liquid
浓度
1.05 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human ZO-1. Blast analysis of the sequence of the immunogen shows 100% identity with human.
靶标信息
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
基因别名: TJP1; ZO-1; ZO1
UniProt ID: (Human) Q07157, (Mouse) P39447
Entrez Gene ID: (Human) 7082, (Mouse) 21872
