Invitrogen

alpha Actinin 1 Polyclonal Antibody

alpha Actinin 1 Antibody in Immunocytochemistry (ICC/IF)

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alpha Actinin 1 Antibody (PA5-17308) in ICC/IF

Immunofluorescent analysis of Alpha-Actinin in NIH/3T3 cells using an Alpha-Actinin polyclonal antibody (Product # PA5-17308) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

alpha Actinin 1 Antibody in Western Blot (WB)

alpha Actinin 1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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产品信息

PA5-17308

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

1:25

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Hamster, Human, Mouse, Non-human primate, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to carboxy-terminal residues of human a-actinin-1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

5 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10986146

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

Alpha Actinin 1 belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer.

Journal of translational medicine

Wang C,Xie B,Yin S,Cao J,Huang J,Jin L,Du G,Zhai X,Zhang R,Li S,Cao T,Yu H,Fan X,Yang Z,Peng J,Xiao J,Lian L

PA5-17308 was used in Immunocytochemistry-immunofluorescence to propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.

Mon Jun 19 00:00:00 UTC 2023

Saffron: A Multitask Neuroprotective Agent for Retinal Degenerative Diseases.

Antioxidants (Basel, Switzerland)

Di Marco S,Carnicelli V,Franceschini N,Di Paolo M,Piccardi M,Bisti S,Falsini B

PA5-17308 was used in Western Blotting to show a way of action of saffron treatment confirming the complex nature of neuroprotective activities of its chemical components.

Wed Jul 17 00:00:00 UTC 2019

Increased free Zn²⁺ correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn²⁺ -transporters in heart failure.

Journal of cellular and molecular medicine

Olgar Y,Durak A,Tuncay E,Bitirim CV,Ozcinar E,Inan MB,Tokcaer-Keskin Z,Akcali KC,Akar AR,Turan B

Published figure using alpha Actinin polyclonal antibody (Product # PA5-17308) in Western Blot

Thu Mar 01 00:00:00 UTC 2018

Generation of two human isogenic iPSC lines from fetal dermal fibroblasts.

Stem cell research

Tandon R,Brändl B,Baryshnikova N,Landshammer A,Steenpaß L,Keminer O,Pless O,Müller FJ

PA5-17308 was used in Immunocytochemistry-immunoflourescence to show that both new cell lines offer the opportunity to study basic stem cell biology and model human disease.

Sat Dec 01 00:00:00 UTC 2018

Variants in exons 5 and 6 of ACTB cause syndromic thrombocytopenia.

Nature communications

Latham SL,Ehmke N,Reinke PYA,Taft MH,Eicke D,Reindl T,Stenzel W,Lyons MJ,Friez MJ,Lee JA,Hecker R,Frühwald MC,Becker K,Neuhann TM,Horn D,Schrock E,Niehaus I,Sarnow K,Grützmann K,Gawehn L,Klink B,Rump A,Chaponnier C,Figueiredo C,Knöfler R,Manstein DJ,Di Donato N

Published figure using alpha Actinin 1 polyclonal antibody (Product # PA5-17308) in Immunofluorescence

Fri Oct 12 00:00:00 UTC 2018

Variants in exons 5 and 6 of ACTB cause syndromic thrombocytopenia.

Nature communications

Published figure using alpha Actinin 1 polyclonal antibody (Product # PA5-17308) in Immunofluorescence

Fri Oct 12 00:00:00 UTC 2018

Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration.

International journal of molecular medicine

Park S,Choi Y,Jung N,Yu Y,Ryu KH,Kim HS,Jo I,Choi BO,Jung SC

PA5-17308 was used in immunocytochemistry and western blot to assess the potential to use myogenic differentiated human tonsil-derived mesenchymal stem cells to promote skeletal muscle regeneration

Sun May 01 00:00:00 UTC 2016

Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration.

International journal of molecular medicine

Park S,Choi Y,Jung N,Yu Y,Ryu KH,Kim HS,Jo I,Choi BO,Jung SC

PA5-17308 was used in immunocytochemistry and western blot to assess the potential to use myogenic differentiated human tonsil-derived mesenchymal stem cells to promote skeletal muscle regeneration

Sun May 01 00:00:00 UTC 2016

Use of Adeno-Associated Virus to Enrich Cardiomyocytes Derived from Human Stem Cells.

Human gene therapy. Clinical development

Guan X,Wang Z,Czerniecki S,Mack D,François V,Blouin V,Moullier P,Childers MK

Published figure using alpha Actinin 1 polyclonal antibody (Product # PA5-17308) in Immunofluorescence

Tue Sep 01 00:00:00 UTC 2015

生物信息学

蛋白别名: actinin 1 smooth muscle; actinin, alpha 1; alpha actinin 1a; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; F-actin cross-linking protein; FLJ40884; FLJ54432; non-muscle alpha-actinin 1; Non-muscle alpha-actinin-1

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基因别名: 3110023F10Rik; ACTN1; Actn1a; BDPLT15; I79_004057

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UniProt ID: (Human) P12814, (Rat) Q9Z1P2, (Mouse) Q7TPR4