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alpha Actinin 1 Antibody (PA5-82233) in ICC/IF
Immunofluorescence analysis of alpha Actinin 1 was performed using 70 confluent log phase HSkMs cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with alpha Actinin 1 Polyclonal Antibody (Product # PA5-82233, 1:100) in 0.1% BSA, incubated at 4 degree cel... View More
产品信息
PA5-82233
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
偶联物
形式
Liquid
浓度
0.05 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 40% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2789394
产品详细信息
Immunogen sequence: SAKEGLLLWC QRKTAPYKNV NIQNFHISWK DGLGFCALIH RHRPELIDYG KLRKDDPLTN LNTAFDVAEK YLDIPKMLDA EDIVGTARPD EKAIMTYVSS FYHAFSGAQK AETAANRICK V
靶标信息
Alpha Actinin 1 belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: actinin 1 smooth muscle; actinin, alpha 1; alpha actinin 1a; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; F-actin cross-linking protein; FLJ40884; FLJ54432; non-muscle alpha-actinin 1; Non-muscle alpha-actinin-1
基因别名: 3110023F10Rik; ACTN1; Actn1a; BDPLT15
UniProt ID: (Human) P12814, (Rat) Q9Z1P2, (Mouse) Q7TPR4
Entrez Gene ID: (Human) 87, (Rat) 81634, (Mouse) 109711
double-stranded RNA binding
integrin binding
calcium ion binding
protein binding
vinculin binding
ligand-dependent nuclear receptor transcription coactivator activity
protein homodimerization activity
ion channel binding
actin filament binding
protein domain specific binding
actin binding
metal ion binding
platelet degranulation
actin filament organization
platelet activation
platelet formation
platelet morphogenesis
regulation of apoptotic process
focal adhesion assembly
actin filament bundle assembly
negative regulation of cellular component movement
actin filament network formation
actin crosslink formation
platelet aggregation
regulation of nucleic acid-templated transcription
cortical cytoskeleton organization
