Invitrogen

alpha Actinin 4 Polyclonal Antibody

alpha Actinin 4 Antibody in Immunocytochemistry (ICC/IF)

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alpha Actinin 4 Antibody (42-1400) in ICC/IF

Immunofluorescence analysis of alpha Actinin 4 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Actinin 4 Rabbit Polyclonal Antibody (Product # 42-1400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at ... View More

alpha Actinin 4 Antibody in Immunohistochemistry (IHC)

alpha Actinin 4 Antibody in Western Blot (WB)

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产品信息

42-1400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-3 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

2-3 µg/mL

免疫细胞化学 (ICC/IF)

2-3 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the N-terminal region of the human, mouse, and orangutan alpha-Actinin-4 protein

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533514

靶标信息

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Increased tissue stiffness triggers contractile dysfunction and telomere shortening in dystrophic cardiomyocytes.

Stem cell reports

Chang ACY,Pardon G,Chang ACH,Wu H,Ong SG,Eguchi A,Ancel S,Holbrook C,Ramunas J,Ribeiro AJS,LaGory EL,Wang H,Koleckar K,Giaccia A,Mack DL,Childers MK,Denning C,Day JW,Wu JC,Pruitt BL,Blau HM

Published figure using alpha Actinin 4 polyclonal antibody (Product # 42-1400) in Immunocytochemistry

Tue Sep 14 00:00:00 UTC 2021

N-sulfation of heparan sulfate is critical for syndecan-4-mediated podocyte cell-matrix interactions.

American journal of physiology. Renal physiology

Sugar T,Wassenhove-McCarthy DJ,Orr AW,Green J,van Kuppevelt TH,McCarthy KJ

42-1400 was used in immunocytochemistry to study syndecan-4-mediated podocyte cell-matrix interactions that require N-sulfation of heparan sulfate

Sun May 15 00:00:00 UTC 2016

Podocytes require the engagement of cell surface heparan sulfate proteoglycans for adhesion to extracellular matrices.

Kidney international

Chen S,Wassenhove-McCarthy D,Yamaguchi Y,Holzman L,van Kuppevelt TH,Orr AW,Funk S,Woods A,McCarthy K

42-1400 was used in immunohistochemistry to study the contribution of proteoglycans to podocyte adhesion

Wed Dec 01 00:00:00 UTC 2010

Interaction of zonula occludens-1 (ZO-1) with alpha-actinin-4: application of functional proteomics for identification of PDZ domain-associated proteins.

Journal of proteome research