Invitrogen

alpha Actinin 4 Monoclonal Antibody (7H6)

alpha Actinin 4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 6

alpha Actinin 4 Antibody (MA1-800) in IHC (P)

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse mono... View More

alpha Actinin 4 Antibody in Western Blot (WB)

Product Image Gallery

产品信息

MA1-800

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

4 µg/mL

流式细胞分析 (Flow)

1 µg / 10^6 cells

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Mouse / IgG

分类

Monoclonal

类型

Antibody

克隆号

7H6

抗原

Synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actinin 4.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2221690

产品详细信息

MA1-800 detects the alpha Actinin 4 in human and mouse cells.

MA1-800 has been successfully used in Western blot, and immunofluorescence procedures. MA1-800 detects alpha Actinin 4 which has a predicted molecular weight of approximately 105 kDa. Under reducing conditions, MA1-800 detects a monomer of alpha Actinin 4 at approximately 52.5 kDa.

The MA1-800 immunogen is a synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actin 4.

靶标信息

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。