Invitrogen

alpha Adaptin Monoclonal Antibody (AC1-M11)

alpha Adaptin Antibody in Immunocytochemistry (ICC/IF)

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alpha Adaptin Antibody (MA3-061) in ICC/IF

Immunofluorescent analysis of alpha Adaptin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Adaptin (AC1-M11) Mouse Monoclonal Antibody (Product # MA3-061) at 2 µg/mL in 0.1% BSA and incubated ... View More

alpha Adaptin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Adaptin Antibody in Western Blot (WB)

alpha Adaptin Antibody in Immunoprecipitation (IP)

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产品信息

MA3-061

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

查看14篇已发表文章 14篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

1:100

查看5篇已发表文章 5篇已发表文章

免疫沉淀 (IP)

Assay-dependent

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Amphibian, Bovine, Chicken, Human, Mouse, Non-human primate, Pig, Rat

已发表种属

Amphibian, Bovine, Human, Mouse, Non-human primate, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

AC1-M11

抗原

Purified bovine brain adaptor complexes.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2056321

产品详细信息

MA3-061 detects alpha-adaptin from bovine, chicken, frog, human, monkey, mouse, pig and rat. This antibody detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons.

MA3-061 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody recognizes an ~100 kDa doublet representing the two isoforms of alpha-adaptin from pig brain extract. Immunofluorescence staining of alpha-adaptin in transfected COS cells with MA3-061A yields distinct punctate dots on the plasma membrane.

The MA3-061 antigen is purified bovine brain adaptor complexes.

靶标信息

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

An internally eGFP-tagged α-adaptin is a fully functional and improved fiduciary marker for clathrin-coated pit dynamics.

Traffic (Copenhagen, Denmark)

Mino RE,Chen Z,Mettlen M,Schmid SL

MA3-061 was used in Western Blotting to generate stable cells lines overexpressing mRuby-CLCa and/or µ2-eGFP, σ2-eGFP, two markers currently in use, or a novel marker generated by inserting eGFP into the unstructured hinge region of the α subunit (α-eGFP).

Tue Sep 01 00:00:00 UTC 2020

Indoleamine 2,3-Dioxygenase 2 Immunohistochemical Expression in Resected Human Non-small Cell Lung Cancer: A Potential New Prognostic Tool.

Frontiers in immunology

Mandarano M,Bellezza G,Belladonna ML,Vannucci J,Gili A,Ferri I,Lupi C,Ludovini V,Falabella G,Metro G,Mondanelli G,Chiari R,Cagini L,Stracci F,Roila F,Puma F,Volpi C,Sidoni A

Published figure using alpha Adaptin monoclonal antibody (Product # MA3-061) in Immunoprecipitation

Mon Mar 15 00:00:00 UTC 2021

Cell-specific non-canonical amino acid labelling identifies changes in the de novo proteome during memory formation.

eLife

Evans HT,Bodea LG,Götz J

MA3-061 was used in Western Blotting to show changes of 156 proteins during spatial memory formation in hippocampal neurons by cell-type-specific labelling of newly synthesised proteins with non-canonical amino acids.

Mon Jan 06 00:00:00 UTC 2020

DASC, a sensitive classifier for measuring discrete early stages in clathrin-mediated endocytosis.

eLife

Wang X,Chen Z,Mettlen M,Noh J,Schmid SL,Danuser G

MA3-061 was used in Western Blotting to show that DASC is a sensitive detector of phenotypic variation in clathrin-coated pit dynamics.

Thu Apr 30 00:00:00 UTC 2020

Mutations in Disordered Regions Can Cause Disease by Creating Dileucine Motifs.

Cell

Meyer K,Kirchner M,Uyar B,Cheng JY,Russo G,Hernandez-Miranda LR,Szymborska A,Zauber H,Rudolph IM,Willnow TE,Akalin A,Haucke V,Gerhardt H,Birchmeier C,Kühn R,Krauss M,Diecke S,Pascual JM,Selbach M

MA3-061 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to study how mutations in disordered cytosolic regions of three transmembrane proteins (GLUT1, ITPR1, and CACNA1H) lead to an increased clathrin binding.

Thu Sep 20 00:00:00 UTC 2018

A chloroquine-induced macrophage-preconditioning strategy for improved nanodelivery.

Scientific reports

Wolfram J,Nizzero S,Liu H,Li F,Zhang G,Li Z,Shen H,Blanco E,Ferrari M

Published figure using alpha Adaptin monoclonal antibody (Product # MA3-061) in Western Blot

Mon Oct 23 00:00:00 UTC 2017

Ikarugamycin: A Natural Product Inhibitor of Clathrin-Mediated Endocytosis.

Traffic (Copenhagen, Denmark)

Elkin SR,Oswald NW,Reed DK,Mettlen M,MacMillan JB,Schmid SL

Published figure using alpha Adaptin monoclonal antibody (Product # MA3-061) in Immunofluorescence

Sat Oct 01 00:00:00 UTC 2016

Ikarugamycin: A Natural Product Inhibitor of Clathrin-Mediated Endocytosis.

Traffic (Copenhagen, Denmark)

Elkin SR,Oswald NW,Reed DK,Mettlen M,MacMillan JB,Schmid SL

Published figure using alpha Adaptin monoclonal antibody (Product # MA3-061) in Immunofluorescence

Sat Oct 01 00:00:00 UTC 2016

Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation.

Nature cell biology

Rong Y,Liu M,Ma L,Du W,Zhang H,Tian Y,Cao Z,Li Y,Ren H,Zhang C,Li L,Chen S,Xi J,Yu L

MA3-061 was used in western blot to study the regulation of autophagic lysosome reformation by clathrin and phosphatidylinositol-4,5-bisphosphate

Sat Sep 01 00:00:00 UTC 2012

Recruitment of endophilin to clathrin-coated pit necks is required for efficient vesicle uncoating after fission.

Neuron

Milosevic I,Giovedi S,Lou X,Raimondi A,Collesi C,Shen H,Paradise S,O'Toole E,Ferguson S,Cremona O,De Camilli P

MA3-061 was used in western blot to investigate the role of endophilin recruitment in vesicle uncoating during endocytosis

Thu Nov 17 00:00:00 UTC 2011

Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Zander JF,Münster-Wandowski A,Brunk I,Pahner I,Gómez-Lira G,Heinemann U,Gutiérrez R,Laube G,Ahnert-Hilger G

MA3-061 was used in western blot to investigate the colocalization of VGLUT and VGAT and its effect on neurotransmitter uptake

Wed Jun 02 00:00:00 UTC 2010

Endocytosis in cultured neurons is altered by chronic alcohol exposure.

Toxicological sciences : an official journal of the Society of Toxicology

Marín MP,Esteban-Pretel G,Ponsoda X,Romero AM,Ballestín R,López C,Megías L,Timoneda J,Molowny A,Canales JJ,Renau-Piqueras J

MA3-061 was used in western blot to investigate the influence of chronic alcohol consumption on neuronal endocytosis

Sat May 01 00:00:00 UTC 2010

Silencing of fas-associated death domain protects mice from septic lung inflammation and apoptosis.

American journal of respiratory and critical care medicine

Matsuda N,Yamamoto S,Takano K,Kageyama S,Kurobe Y,Yoshihara Y,Takano Y,Hattori Y

MA3-061 was used in western blot to investigate the effects of siRNA-mediated silencing of fas-associated death domain (FADD) on septic lung inflammation and apoptosis

Fri May 01 00:00:00 UTC 2009

ARAP1 regulates endocytosis of EGFR.

Traffic (Copenhagen, Denmark)

Yoon HY,Lee JS,Randazzo PA

MA3-061 was used in immunocytochemistry to investigate the effect of ARAP1 on endocytosis of EGFR

Mon Dec 01 00:00:00 UTC 2008

A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis.

Science (New York, N.Y.)

Ferguson SM,Brasnjo G,Hayashi M,Wölfel M,Collesi C,Giovedi S,Raimondi A,Gong LW,Ariel P,Paradise S,O'toole E,Flavell R,Cremona O,Miesenböck G,Ryan TA,De Camilli P

MA3-061 was used in immunocytochemistry to investigate the role of dynamin 1 in nervous system function

Fri Apr 27 00:00:00 UTC 2007

Death-receptor activation halts clathrin-dependent endocytosis.

Proceedings of the National Academy of Sciences of the United States of America

Austin CD,Lawrence DA,Peden AA,Varfolomeev EE,Totpal K,De Mazière AM,Klumperman J,Arnott D,Pham V,Scheller RH,Ashkenazi A

Published figure using alpha Adaptin monoclonal antibody (Product # MA3-061) in Immunoprecipitation

Wed Jul 05 00:00:00 UTC 2006

Interaction of SPIN90 with syndapin is implicated in clathrin-mediated endocytic pathway in fibroblasts.

Genes to cells : devoted to molecular & cellular mechanisms

Kim SH,Choi HJ,Lee KW,Hong NH,Sung BH,Choi KY,Kim SM,Chang S,Eom SH,Song WK

MA3-061 was used in immunohistochemistry to investigate the interaction of SPIN90 and syndapin and its role in clathrin-mediated endocytic pathway

Sun Oct 01 00:00:00 UTC 2006

Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment.

Molecular biology of the cell

Ivanov AI,Nusrat A,Parkos CA

MA3-061 was used in immunocytochemistry to investigate the mechanism of endocytosis of adherens junction proteins and tight junction proteins in T84 epithelial cells

Thu Jan 01 00:00:00 UTC 2004

Tensile stress induces alpha-adaptin C production in mouse calvariae in an organ culture: possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation.

Journal of cellular physiology

Shimomura J,Ishibashi O,Ikegame M,Yoshizawa T,Ejiri S,Noda T,Kawashima H

MA3061 was used in western blot to explore the effects of tensile stress on osteoblast differentiation and osteogenesis

Sun Jun 01 00:00:00 UTC 2003

Xenopus autosomal recessive hypercholesterolemia protein couples lipoprotein receptors with the AP-2 complex in oocytes and embryos and is required for vitellogenesis.

The Journal of biological chemistry

Zhou Y,Zhang J,King ML

MA3-061 was used in western blot to demonstrate the function of xARH in the interactions among LDL, vitellogenin receptors, and the AP-2 complex.

Fri Nov 07 00:00:00 UTC 2003

Cytoplasmic transport of Stat3 by receptor-mediated endocytosis.

The EMBO journal

Bild AH,Turkson J,Jove R

Published figure using alpha Adaptin monoclonal antibody (Product # MA3-061) in Immunofluorescence

Mon Jul 01 00:00:00 UTC 2002

A beta-turn endocytic code is required for optimal internalization of the growth hormone receptor but not for alpha-adaptin association.

Molecular endocrinology (Baltimore, Md.)

Vleurick L,Pezet A,Kühn ER,Decuypere E,Edery M

MA3-061 was used in western blot to study the mechanism for the internalization of the growth hormone receptor.

Mon Nov 01 00:00:00 UTC 1999

100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties.

The Journal of cell biology

Wong DH,Brodsky FM

MA3-061 was used in western blot to study coat protein membrane binding properties in the Golgi region

Mon Jun 01 00:00:00 UTC 1992

生物信息学

蛋白别名: 100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; Adaptor protein complex AP-2 subunit alpha-1; adaptor protein complex AP-2, alpha 1 subunit; adaptor related protein complex 2, alpha 1 subunit; Adaptor-related protein complex 2 subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; adaptor-related protein complex AP-2, alpha 1 subunit; ADTAB; Alpha-adaptin A; alpha-adaptin C; Alpha1-adaptin; alpha2-adaptin; AP-2 complex subunit alpha-1; AP2; CLAPA2; Clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; HIP-9; HIP9; HYPJ; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

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基因别名: ADTAA; AP2-ALPHA; AP2A1; BOS_17797; CLAPA1

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UniProt ID: (Human) O95782, (Mouse) P17426

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Entrez Gene ID: (Bovine) 784636, (Human) 160, (Rat) 308578, (Mouse) 11771

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功能

protein binding protein C-terminus binding protein transporter activity protein kinase binding protein complex binding clathrin adaptor activity

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