Invitrogen

alpha COP Polyclonal Antibody

alpha COP Antibody in Immunocytochemistry (ICC/IF)

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alpha COP Antibody (PA1-067) in ICC/IF

Immunolocalization of COP alpha in HeLa cells using Product # PA1-067.

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产品信息

PA1-067

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫细胞化学 (ICC/IF)

3 µg/mL

免疫沉淀 (IP)

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产品规格

种属反应

Human, Rat

已发表种属

Human, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2081286

产品详细信息

PA1-067 detects coatomer-protein I alpha (COPI alpha) from human and rat cells.

PA1-067 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~140 kDa protein representing COP alpha from PC-12 cell extract. This antibody also detects an unidentified protein at ~100 kDa. Immunocytochemical staining of alpha-COP using PA1-067 results in alpha-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm.

PA1-067 immunogen is a synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP. The PA1-067 immunizing peptide (Cat. # PEP-125) is available for use in neutralization and control experiments.

靶标信息

In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。