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Invitrogen
alpha GalCer:CD1d Complex Monoclonal Antibody (L363), PE, eBioscience™
FIGURE: 1 / 4
alpha GalCer:CD1d Complex Antibody (12-2019-82) in Flow
Staining of mouse CD1d-transfected cells loaded overnight with alpha-galactosylceramide (right) or left untreated (left) with 0.25 µg of Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (blue histogram) or 0.125 µg of Anti-Mouse alpha-GalCer:CD1d Complex PE (purple histogram). Total viable cells were used for analysis.
Product Details
12-2019-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
L363
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1944355
Product Specific Information
Description: This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.
The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes.
Applications Reported: This L363 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This L363 antibody has been tested by flow cytometric analysis of alpha-galactosylceramide-loaded mouse CD1d-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Va14Ja18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase; 3'-phosphoadenylylsulfate-galactosylceramide 3'-sulfotransferase; 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase; Antigen-presenting glycoprotein CD1d1; CD1d.1; Cerebroside sulfotransferase; Galactosylceramide sulfotransferase; GalCer sulfotransferase; T-cell surface glycoprotein CD1d1
Gene Aliases: AI747460; CD1.1; Cd1a; Cd1d; Cd1d1; Cst; Gal3st1; Gcst; Ly-38
UniProt ID: (Mouse) P11609, (Mouse) Q9JHE4
Entrez Gene ID: (Mouse) 12479, (Mouse) 53897
protein binding
beta-2-microglobulin binding
lipid antigen binding
endogenous lipid antigen binding
exogenous lipid antigen binding
histone binding
T cell receptor binding
cell adhesion molecule binding
lipopeptide binding
galactosylceramide sulfotransferase activity
transferase activity
major histocompatibility complex protein
transferase
positive regulation of T cell mediated cytotoxicity
immune system process
immune response
antigen processing and presentation
positive regulation of T cell proliferation
positive regulation of macrophage activation
positive thymic T cell selection
positive regulation of interferon-gamma biosynthetic process
positive regulation of interleukin-2 biosynthetic process
innate immune response
positive regulation of interleukin-4 biosynthetic process
antigen processing and presentation, endogenous lipid antigen via MHC class Ib
antigen processing and presentation, exogenous lipid antigen via MHC class Ib
regulation of immune response
positive regulation of NK T cell activation
positive regulation of NK T cell differentiation
lipid metabolic process
sphingolipid metabolic process
galactosylceramide biosynthetic process
spermatogenesis
biosynthetic process
glycolipid biosynthetic process
myelination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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