Invitrogen

alpha GalCer:CD1d Complex Monoclonal Antibody (L363), PE, eBioscience™

alpha GalCer:CD1d Complex Antibody in Flow Cytometry (Flow)

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alpha GalCer:CD1d Complex Antibody (12-2019-82) in Flow

Staining of mouse CD1d-transfected cells loaded overnight with alpha-galactosylceramide (right) or left untreated (left) with 0.25 µg of Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (blue histogram) or 0.125 µg of Anti-Mouse alpha-GalCer:CD1d Complex PE (purple histogram). Total viable cells were used for analysis.

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产品信息

12-2019-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

L363

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1944355

产品详细信息

Description: This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.

The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes.

Applications Reported: This L363 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This L363 antibody has been tested by flow cytometric analysis of alpha-galactosylceramide-loaded mouse CD1d-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Va14Ja18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Nanocell COVID-19 vaccine triggers a novel immune response pathway producing high-affinity antibodies which neutralize all variants of concern.

Frontiers in immunology

Gao SY,Amaro-Mugridge NB,Madrid-Weiss J,Petkovic N,Vanegas N,Visvanathan K,Williams BRG,MacDiarmid JA,Brahmbhatt H

12-2019-82 was used in Flow cytometry/Cell sorting to show for the first time in mice and humans, that a bacterially derived, non-living, nanocell (EDV; EnGeneIC Dream Vector) packaged with plasmid expressed SARS-CoV-2 S-protein and α-galactosyl ceramide adjuvant (EDV-COVID-αGC), stimulates an alternate pathway due to dendritic cells (DC) displaying both S-polypeptides and αGC thereby recruiting and activating iNKT cells with release of IFNγ.

Mon Feb 27 00:00:00 UTC 2023

CD1d highly expressed on DCs reduces lung tumor burden by enhancing antitumor immunity.

Oncology reports

Li Y,Zhao C,Liu J,Lu Z,Lu M,Gu J,Liu R

12-2019 was used in Flow cytometry/Cell sorting to investigate whether CD1d expression on dendritic cells is capable of enhancing anti-tumour immunity, particularly T-cell related immunity.

Wed May 01 00:00:00 UTC 2019

Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae.

Nature communications

Chandra S,Gray J,Kiosses WB,Khurana A,Hitomi K,Crosby CM,Chawla A,Fu Z,Zhao M,Veerapen N,Richardson SK,Porcelli SA,Besra G,Howell AR,Sharma S,Peters B,Kronenberg M

Mon Oct 15 00:00:00 UTC 2018

The autophagy machinery restrains iNKT cell activation through CD1D1 internalization.

Autophagy

Keller CW,Loi M,Ewert S,Quast I,Theiler R,Gannagé M,Münz C,De Libero G,Freigang S,Lünemann JD

12-2019-82 was used in Flow Cytometry to indicate that the autophagic machinery assists in the recruitment of AP2 to CD1D1 molecules resulting in attenuated iNKT cell activation, in contrast to the supporting role of macroautophagy in CD4+ T cell stimulation.

Sat Jun 03 00:00:00 UTC 2017

生物信息学

蛋白别名: 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase; 3'-phosphoadenylylsulfate-galactosylceramide 3'-sulfotransferase; 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase; Antigen-presenting glycoprotein CD1d1; CD1d.1; Cerebroside sulfotransferase; Galactosylceramide sulfotransferase; GalCer sulfotransferase; T-cell surface glycoprotein CD1d1

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基因别名: AI747460; CD1.1; Cd1a; Cd1d; Cd1d1; Cst; Gal3st1; Gcst; Ly-38

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UniProt ID: (Mouse) P11609, (Mouse) Q9JHE4

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Entrez Gene ID: (Mouse) 12479, (Mouse) 53897

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功能