Invitrogen

alpha GalCer:CD1d Complex Monoclonal Antibody (L363), Functional Grade, eBioscience™

alpha GalCer:CD1d Complex Antibody in Flow Cytometry (Flow)

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alpha GalCer:CD1d Complex Antibody (16-2019-82) in Flow

Oncology reports 2019 - Figure 5. (A) The correlation analysis between CD1d + DC cell score and Co antigen-presenting activity score. The costimulatory molecule scores were calculated by combining the expression levels of CD40, CD80 and CD86; the MHC-I score was calculated by combining the expression levels of HLA-A/B/C; and the MHC-II score was calculated by combining the expression levels of HLA-DMA/DOA/DQA1/DRA. The co antigen-... View More

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产品信息

16-2019-82

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.25 µg/test

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功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

L363

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_1944353

产品详细信息

Description: This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.

The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes.

Applications Reported: This L363 antibody has been reported for use in functional assays.

Applications Tested: This L363 antibody has been tested by flow cytometric analysis of alpha-galactosylcermide-loaded mouse CD1d-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Va14Ja18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CD1d highly expressed on DCs reduces lung tumor burden by enhancing antitumor immunity.

Oncology reports

Li Y,Zhao C,Liu J,Lu Z,Lu M,Gu J,Liu R

Published figure using alpha GalCer:CD1d Complex monoclonal antibody (Product # 16-2019-82) in Flow Cytometry

Wed May 01 00:00:00 UTC 2019

Abrogation of Endogenous Glycolipid Antigen Presentation on Myelin-Laden Macrophages by D-Sphingosine Ameliorates the Pathogenesis of Experimental Autoimmune Encephalomyelitis.

Frontiers in immunology

Ban Y,Dong W,Zhang L,Zhou T,Altiti AS,Ali K,Mootoo DR,Blaho VA,Hla T,Ren Y,Ma X

16-2019 was used in Flow cytometry/Cell sorting to reveal a novel pathway from the presentation of Mye-GalCer to IL-17 production, and highlight the promising therapeutic potential of D-sphingosine for the human disorder of multiple sclerosis.

Tue Jul 14 00:00:00 UTC 2020

Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae.

Nature communications

Chandra S,Gray J,Kiosses WB,Khurana A,Hitomi K,Crosby CM,Chawla A,Fu Z,Zhao M,Veerapen N,Richardson SK,Porcelli SA,Besra G,Howell AR,Sharma S,Peters B,Kronenberg M

Published figure using alpha GalCer:CD1d Complex monoclonal antibody (Product # 16-2019-82) in Flow Cytometry

Mon Oct 15 00:00:00 UTC 2018

Mutation of a positively charged cytoplasmic motif within CD1d results in multiple defects in antigen presentation to NKT cells.

Journal of immunology (Baltimore, Md. : 1950)

Shin JH,Park JY,Shin YH,Lee H,Park YK,Jung S,Park SH

16-2019-82 was used in Immunocytochemistry to demonstrate that the (327-329)RRR motif of CD1d is required for cellular distribution of CD1d and optimal Ag presentation.

Thu Mar 01 00:00:00 UTC 2012

Kinetics and cellular site of glycolipid loading control the outcome of natural killer T cell activation.

Immunity

Im JS,Arora P,Bricard G,Molano A,Venkataswamy MM,Baine I,Jerud ES,Goldberg MF,Baena A,Yu KO,Ndonye RM,Howell AR,Yuan W,Cresswell P,Chang YT,Illarionov PA,Besra GS,Porcelli SA

Published figure using alpha GalCer:CD1d Complex monoclonal antibody (Product # 16-2019-82) in Flow Cytometry

Fri Jun 19 00:00:00 UTC 2009

生物信息学

蛋白别名: 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase; 3'-phosphoadenylylsulfate-galactosylceramide 3'-sulfotransferase; 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase; Antigen-presenting glycoprotein CD1d1; CD1d.1; Cerebroside sulfotransferase; Galactosylceramide sulfotransferase; GalCer sulfotransferase; T-cell surface glycoprotein CD1d1

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基因别名: AI747460; CD1.1; Cd1a; Cd1d; Cd1d1; Cst; Gal3st1; Gcst; Ly-38

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UniProt ID: (Mouse) P11609, (Mouse) Q9JHE4

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Entrez Gene ID: (Mouse) 12479, (Mouse) 53897

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功能